Rhizosheric bacteria
AssigneeResearch Seeds, Inc.
InventorsClive W. Ronson, Robert W. Kwiatkowski
Life of this patent
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Abstract
A bacterial inoculum including a rhizospheric bacterium with increased dicarboxylic acid membrane permease activity.
Claims14
A method for increasing occupancy of plant nodules in soil or vermiculite with modified Rhizobium or Bradyrhizobium bacteria, comprising:
- making modified Rhizobium or Bradyrhizobium bacteria having, in addition to endogenous dctA, B, and D genes, an exogenous DNA sequence comprising a dctA gene under the control of an nptII promoter;
- adding a seed coating material to the bacteria to form a mixture;
- coating seeds of nodulating plants with the mixture;
- and planting said seeds in soil or vermiculite.
A method for increasing occupancy of plant nodules in soil or vermiculite with modified Rhizobium or Bradyrhizobium bacteria, comprising:
- making modified Rhizobium or Bradyrhizobium bacteria having at least two DNA sequences encoding a dctA gene product, wherein expression of one of the DNA sequences is controlled by a constitutive promoter;
- adding a seed coating material to the bacteria to form a mixture;
- coating seeds of nodulating plants with the mixture;
- and planting said seeds in soil or vermiculite.
A composition for increasing occupancy of plant nodules in soil or vermiculite with modified Rhizobium or Bradyrhizobium bacteria, comprising an agriculturally acceptable carrier in combination with either Rhizobium or Bradyrhizobium bacteria which have been modified to have, in addition to endogenous dctA, B, and D genes, an exogenous DNA sequence comprising a dctA gene under the control of an nptII promoter.
Dependencies are inferred from the claim text.