Kit for detection of islet amyloid polypeptide (IAPP)
AssigneeRegents of the University of Minnesota
InventorsPer Westermark, Kenneth H. Johnson
Life of this patent
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Abstract
This invention is directed to kits for the detection of human islet amyloid polypeptide (IAPP) comprising (a) purified preparations of antibodies which react specifically with insulin or calcitonin gene-related peptides and (b) a preselected amount of human islet amyloid polypeptide which is essentially free of islet amyloid, which polypeptide is one subunit of islet amyloid and which is prepared by depolymerizing human islet amyloid; or a preselected amount of human islet amyloid polypeptide which is essentially free of islet amyloid and has the amino acid sequence: lys-cys-Asn-Thr-Ala-Thr-Cys-Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Val-His-Se r-Ser-Asn-Asn-Phe-Gly-Ala-Ile-Leu-Ser-Ser-Thr-Asn-Val-Gly-Ser-Asn-Thr-Tyr.
Claims13
An assay kit for the detection of human islet amyloid polypeptide (IAPP) which comprises (a) a purified preparation of polyclonal antibodies or monoclonal antibodies which reacts specifically with human islet amyloid polypeptide, which polypeptide is one subunit of islet amyloid and which is prepared by depolymerizing human islet amyloid, wherein said antibody does not significantly react with insulin or with calcitonin gene-related peptides selected from the group consisting of human CGRP1 and human CGRP2;
- and (b) a preselected amount of said human islet amyloid polypeptide.
An assay kit for the detection of human islet amyloid polypeptide (IAPP) which comprises (a) a purified preparation of polyclonal antibodies or monoclonal antibodies which reacts specifically with a polypeptide of the amino acid sequence:
- ##STR3## wherein said antibodies do not significantly react with insulin or calcitonin gene-related peptides selected from the group consisting of human CGRP1 and human CGRP2;
- and (b) a preselected amount of human islet amyloid polypeptide essentially free of islet amyloid, said human islet amyloid polypeptide having the amino acid sequence:
- ##STR4##
An assay kit for the detection of human islet amyloid polypeptide (IAPP) which comprises (a) a purified preparation of polyclonal antibodies or monoclonal antibodies which reacts specifically with a polypeptide of the amino acid sequence:
- Cys-Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Ile,
- wherein said antibodies do not significantly react with insulin or calcitonin gene-related peptides selected from the group consisting of human CGRP1 and human CGRP2;
- and (b) a preselected amount of human polypeptide, which polypeptide is one subunit of islet amyloid and which is prepared by depolymerizing human islet amyloid;
- or a preselected amount of human islet amyloid polypeptide essentially free of islet amyloid, human islet amyloid polypeptide having the amino acid sequence:
- ##STR5##
Dependencies are inferred from the claim text.