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AssigneeUniversity of Florida
InventorsLonnie O. Ingram, David S. Beall, Gerhard F. H. Burchhardt, Walter V. Guimaraes, Kazuyoshi Ohta, Brent E. Wood, Keelnatham T. Shanmugam
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Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.
A gram negative recombinant host, other than Escherichia coli, comprising a first heterologous DNA coding for alcohol dehydrogenase and pyruvate decarboxylase, wherein said host expresses said heterologous DNA at a sufficient functional level so as to facilitate the production of ethanol as a primary fermentation product by said host.
A plasmid comprising genes coding for alcohol dehydrogenase and pyruvate decarboxylase, wherein said plasmid is capable of directing a gram negative host to produce alcohol dehydrogenase and pyruvate decarboxylase at a sufficient functional level to facilitate the production of ethanol as the primary fermentation product by said host.
A method for the production of ethanol, comprising transforming an appropriate gram negative host, other than Escherichia coli, with heterologous genes coding for pyruvate decarboxylase and alcohol dehydrogenase, wherein said host expresses said genes at a sufficient functional level to facilitate the production of ethanol as the primary fermentation product by said host.
A method for reducing the accumulation of acidic metabolic products in the growth medium of gram negative cells, said method comprising transforming said cells with heterologous genes coding for alcohol dehydrogenase and pyruvate decarboxylase, wherein said cells express said genes at a sufficient functional level to facilitate the production of ethanol as the primary fermentation product by said cells.
A gram negative recombinant host, other than E. coli, comprising a first heterologous DNA coding for alcohol dehydrogenase and pyruvate decarboxylase, respectively, wherein said host
A gram negative recombinant host, comprising a first heterologous DNA coding for alcohol dehydrogenase and pyruvate decarboxylase, respectively, wherein said host
A gram negative recombinant host, comprising a first heterologous DNA coding for alcohol dehydrogenase and pyruvate decarboxylase, respectively, wherein said host
Dependencies are inferred from the claim text.