# US 5,424,068 A · Method for immunization of mammals against atherosclerosis and pharmaceutical compositions for obtaining said immunization > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,424,068 A - Document ID: US5424068A - Status: Granted - Kind: A - Application number: 08/027,502 - Filed: 1993-03-05 - Granted: 1995-06-13 - Earliest claimed: 1992-12-07 - Assignees: P. Doina International Ltd. - Inventors: Doina Filip - Web page: https://patentgrep.com/patent/US5424068A ## CPC - A61K 39/0012: {Lipids; Lipoproteins} - C07K 14/775: Apolipopeptides - A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract Pharmaceutical compositions and a method for immunization of mammals against atherosclerosis including steps of a) obtaining blood from a mammal; b) isolating low density lipoprotein from the blood; c) fusing the lipoprotein in vitro to obtain heavy microemulsion lipoprotein fusion particles having new antigenic determinants; d) administering the fusion particles obtained in step (c) to a mammal; and e) allowing the immune system to clear fusion lipoprotein particles from the mammal. ## Claims ### Claim 1 A method for immunization of mammals against atherosclerosis comprising the steps of: a) obtaining blood from said mammal; b) isolating low density lipoprotein from said blood; c) fusing said lipoprotein in vitro to obtain heavy microemulsion lipoprotein fusion particles having a flotation density of 1.20-1.24 g/ml and having new antigenic determinants; d) administering said fusion particles obtained in step (c) to a mammal; e) allowing the removal of heavy microemulsion particles from atherosclerotic lesions by immune mechanisms from said mammal. ### Claim 2 (depends on 1) A method for immunizing mammals against atherosclerosis according to claim 1, wherein said mammal is a human. ### Claim 3 (depends on 1) A method for immunizing mammals against atherosclerosis according to claim 1, wherein the step of fusing said lipoprotein comprises adding a surface active peptide with amphipathic properties to the lipoprotein and then removing said surface active peptide with amphipathic properties. ### Claim 4 (depends on 3) A method for immunizing mammals against atherosclerosis according to claim 3, wherein said surface active peptide with amphipathic properties is bacitracin or vasopressin. ### Claim 5 (depends on 3) A method for immunizing mammals against atherosclerosis according to claim 3, wherein said surface active peptide with amphipathic properties is removed by dialysis. ### Claim 6 (depends on 1) A method for immunizing mammals against atherosclerosis according to claim 1, wherein the step of fusing said lipoprotein comprises inducing the fusion process by osmotic stress to achieve osmoelastic coupling. ### Claim 7 (depends on 1) A method for immunizing mammals against atherosclerosis according to claim 1, wherein the step of fusing said lipoprotein further comprises carrying out fusion in the presence of a fusion buffer. ### Claim 8 (depends on 1) A method for immunizing mammals against atherosclerosis according to claim 1, wherein said heavy microemulsion particles have dimensions between 30 and 300 nm. ### Claim 9 A pharmaceutical composition for the treatment of atherosclerosis and for prophylaxis of atherosclerosis comprising as an active ingredient a reorganized lipid material comprising heavy microemulsion particles having a flotation density of 1.20-1.24 g/ml and having new antigenic determinants which originated from the animal to be treated, and a conventional immunization adjuvant product. ### Claim 10 A method for the preparation of a pharmaceutical composition for the treatment of atherosclerosis and for the prophylaxis of atherosclerosis comprising as an active ingredient a reorganized lipid material comprising heavy microemulsion particles having a flotation density of 1.20-1.24 g/ml and having new antigenic determinants which originated from the animal to be treated, and a conventional immunization adjuvant product comprising the steps: a) obtaining a fusion lipoprotein by adding an amphipathic material to a patient's lipoprotein in a fusion buffer in vitro to fuse said lipoprotein; and b) removing said amphipathic material to isolate said lipoprotein fusion product comprising heavy microemulsion particles having a flotation density of 1.20-1.24 g/ml at having new antigenic determinants and transfer it to a physiological saline by dialysis. ### Claim 11 (depends on 10) A method for the preparation of a pharmaceutical composition according to claim 10 wherein said reorganized lipid material is obtained by inducing a fusion process by osmotic stress to achieve osmoelastic coupling. ### Claim 12 (depends on 9) A method for the treatment of atherosclerosis in mammals by an immunization scheme comprising administering the pharmaceutical composition of claim 9 to a patient's body at two week intervals. ### Claim 13 (depends on 12) A method according to claim 12, wherein said composition is administered by injection. ### Claim 14 (depends on 9) A pharmaceutical composition according to claim 9, wherein said pharmaceutical composition is a vaccine.