Vaccines containing avirulent phop-type microorganisms
AssigneeWashington University
InventorsRoy Curtiss, III, Jorge Galan
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Abstract
The phoP gene and its equivalents are of a type which have "global regulation of pathogenicity", i.e., they coordinately regulate a number of genes including those that encode bacterial virulence factors. In Salmonella, the phoP gene product also controls the expression of non-specific acid phosphatase from the phoN gene. A central feature of the invention are microorganisms which are avirulent as a result, in whole or in part, of a mutation in phoP, but which retain their immunogenicity. These cells are suitable as components of live vaccines.
Claims10
A vaccine for immunizing an individual against disease symptoms caused by Salmonella, said vaccine being comprised of avirulent Salmonella which contain a mutation in the phoP gone, wherein said avirulent Salmonella are unable to cause Salmonella-based disease symptoms and able to colonize in lymphoid tissue for a sufficient time to induce antibody and cellular immunity, and wherein said avirulent Salmonella retain the properties of avirulence and immunogenicity of a Salmonella strain selected from the group consisting of ATCC number 53864, ATCC number 53865, and ATCC number 53866.
A vaccine for immunizing an individual against disease caused by a pathogenic microorganism, wherein said vaccine is comprised of carrier Salmonella cells which contain a mutation in the phoP gene which renders said carrier Salmonella cells avirulent and immunogenic, wherein said carrier Salmonella cells are transformed with a recombinant expression vector encoding an immunogenic antigen from said pathogenic microorganism, and wherein said carrier Salmonella are unable to cause Salmonella-based disease symptoms and able to colonize in lymphoid tissue for a sufficient time to induce antibody and cellular immunity, and wherein said carrier Salmonella retain the properties of avirulence and immunogenicity of a Salmonella strain selected from the group consisting of ATCC number 53864, ATCC number 53865, and ATCC number 53866.
A method of making a vaccine which comprises admixing (1) an immunologically effective dose of avirulent Salmonella which contain a mutation in the phoP gene wherein said avirulent Salmonella are unable to cause Salmonella-based disease symptoms and able to colonize in lymphoid tissue for a sufficient time to induce antibody and cellular immunity, and wherein said avirulent Salmonella retain the properties of avirulence and immunogenicity of a Salmonella strain selected from the group consisting of ATCC number 53864, ATCC number 53865, and ATCC number 53866 with (2) a pharmaceutically acceptable carrier.
A method of making a vaccine which comprises admixing (1) an immunologically effective dose of carrier Salmonella cells which are avirulent and immunogenic wherein said carrier Salmonella cells contain a mutation in the phoP gene, wherein said carrier Salmonella cells are transformed with a recombinant expression vector encoding an immunogenic antigen from a pathogenic microorganism, and wherein said carrier Salmonella are unable to cause Salmonella-based disease symptoms and able to colonize in lymphoid tissue for a sufficient time to induce antibody and cellular immunity, and wherein said carrier Salmonella retain the properties of avirulence and immunogenicity of a Salmonella strain selected from the group consisting of ATCC number 53864, ATCC number 53865, and ATCC number 53866 with (2) a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.