Cell fixative composition and method of staining cells without destroying the cell surface
AssigneeOrtho Diagnostic Systems Inc.
InventorsMark C. Connelly, Utpal R. Chakraborty, Houston G. Brooks, Jr.
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Abstract
A cell fixative composition for fixing the internal components of a cell without disrupting the cell surface components is disclosed. The composition contains a compound for preserving intracellular morphology, a compound for fixing internal components of the cell, a compound which facilitates transportation of components across the cellular membrane, and a compound for increasing permeability of cell membranes. Methods for analyzing cells which have been fixed using the composition are also disclosed.
Claims3
A fixative composition comprising:
- (i) a first fixative compound selected from the group consisting of 2,4-Dinitrobenzene sulfonamides, Dinitrophenols, 3,5-Dinitrosalicylic acid, 2,4-Dinitrobenzoic acid, 5-Sulfosalicylic acid, 2,5-Dihydroxy-1,4-benzene disulfonic acid, 3,5-Dinitrobenzoic acid, 8-Hydroxyquinoline-5-sulfonic acid, 4-Nitrophenol, 3,5-Dinitrosalicylaldehyde, 3,5-Dinitroaniline, Paratoluene sulfonic acid, 2-Mesitylene sulfonic acid, 2-(Trifluoromethyl) benzoic acid, and 2,4-Dinitrobenzene sulfonic acid;
- (ii) an alcohol-free, second fixative compound selected from the group consisting of formaldehyde, paraformaldehyde, glutaraldehyde, acrolein, glyoxal, malonaldehyde, diacetyl, polyaldehydes, carbodiimides, diisocyanates, diazonium compounds, diimido esters, diethylpyrocarbonate, maleimides, benzoquinone, and metallic ions;
- (iii) a fusogenic compound selected from the group consisting of dimethylsulfoxide, sulfolane, 1-methyl-2-pyrrolidinone, polyethylene glycol, and ethyleneglycol;
- and
- (iv) a zwitterionic or non-ionic surfactant.
A fixative composition comprising 2,4-dinitrobenzene sulfonic acid, formaldehyde, dimethyl sulfoxide, and a zwitterionic or non-ionic surfactant.
Dependencies are inferred from the claim text.