# US 5,422,274 A · Internal deletion mutants of soluble T4(CD4)

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,422,274 A
- Document ID: US5422274A
- Status: Granted
- Kind: A
- Application number: 07/830,489
- Filed: 1992-02-05
- Granted: 1995-06-06
- Earliest claimed: 1986-08-21
- Assignees: The Trustees of Columbia University in the City of New York; SmithKline Beckman Corporation
- Inventors: Paul J. Maddon; Richard Axel; Raymond W. Sweet; James Arthos
- Web page: https://patentgrep.com/patent/US5422274A

## CPC

- C12Q 1/703: {Viruses associated with AIDS}
- A61P 31/12: Antivirals
- C07K 14/70514: {CD4}
- C07K 16/2812: {against CD4}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- Y10S 435/974: Aids related test
- Y10S 530/821: Separation of nucleic acid
- Y10S 930/221: Retrovirus related, or human immunodeficiency virus related, or simian immunodeficiency virus related
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

This invention provides a therapeutic agent capable of specifically forming a complex with human immunodeficiency virus envelope glycoprotein which comprises a polypeptide. In one embodiment of the invention, the amino acid sequence of the polypeptide has the amino acid sequence shown in FIG. 6 from about +1 to about +185 fused to the amino acid sequence from about +353 to about +371. In another embodiment of the invention, the amino acid sequence of the polypeptide has the amino acid sequence shown in FIG. 6 from about +1 to about +106 fused to the amino acid sequence from about +353 to about +371. In yet a further embodiment of the invention, the amino acid sequence of the polypeptide has the amino acid sequence shown in FIG. 6 from about +1 to about +185. This invention also provides a method for treating a subject infected with a human immunodeficiency virus. The method treats the subject with an effective amount of a pharmaceutical composition having an effective amount of a therapeutic agent of the invention and a pharmaceutically acceptable carrier.

## Claims

### Claim 1

An expression vector encoding a peptide consisting of the amino acid sequence; lys lys val val gly leu lys gly asp thr val flu leu thr cys thr ala ser gln lys lys ser ile gln phe his trp lys asn ser asn gln ils lys ile leu gly asn gln gly ser phe leu thr lys gly pro ser lys leu asn asp arg ala asp ser arg arg ser leu trp asp gln gly asn phe pro leu ile ile lys asn leu lys ile glu asp ser asp thr tyr ile cys glu val glu asp gln lys glu glu val gln leu leu val phe gly leu thr ala asn ser asp thr his leu leu gln gly gln ser leu thr leu thr leu glu ser pro pro gly ser ser pro ser val gln cys arg ser pro arg gly lys ash ile gln gly gly lys thr leu ser val ser gln leu glu leu gln asp ser gly thr trp thr cys thr val leu gln asn gln lys lys val glu phe lys ile asp ile val val leu ala phe gln lys ala ser ser ile val leu leu glu ser asn ile lys val leu pro thr trp ser thr pro val gln pro.

### Claim 2

An expression vector encoding a peptide consisting of the amino acid sequence: lys lys val val leu gly lys lys gly asp thr val glu leu thr cys thr ala ser gln lys lys ser ile gln phe his trp lys asn ser asn gln ile lys ile leu gly asn gln gly ser phe leu thr lys gly pro ser lys leu asn asp arg ala asp ser arg arg ser leu trp asp gln gly asn phe pro leu ile ile lys asn leu lys ile glu asp ser asp thr tyr ile cys glu val glu asp gln lys glu glu val gln leu leu val phe gly leu thr ala asn ser asp thr val leu leu glu ser asn ile lys val leu pro thr trp ser thr pro val gln pro.

### Claim 3

An expression vector encoding a peptide consisting of the amino acid sequence; lys lys val val leu gly lys lys gly asp thr val glu leu thr cys thr ala ser gln lys lys ser ile gln phe his trp lys asn ser asn gln ile lys ile leu gly asn gln gly ser phe leu thr lys gly pro ser lys leu asn asp arg ala asp ser arg arg ser leu trp asp gln gly asn phe pro leu ile ile lys asn leu lys ile glu asp ser asp thr tyr ile cys glu val glu asp gln lys glu glu val gln leu leu val phe gly leu thr ala asn ser asp thr his leu leu gln gly gln ser leu thr leu thr leu glu ser pro pro gly ser ser pro ser val gln cys arg ser pro arg gly lys asn ile gln gly gly lys thr leu ser val ser gln leu glu leu gln asp ser gly thr trp thr cys thr val leu gln asn gln lys lys val glu phe lys ile asp ile val val leu ala phe gln lys ala ser ser ile.

### Claim 4 (depends on 1, 2, 3)

A Chinese hamster ovary host cell comprising the expression vector of claim 1, 2 or 3.