US 5,422,273 A · Cell collection apparatus

Abstract

An apparatus for use in the efficient collection of cell samples from a cytology brush device. The device is a cylindrical container capable of containing a solution. The open end is sealable with a removable cap. The closed end of the container is formed as a conically shaped end and disposed within the conically shaped closed end is a series of fins. The fins are oriented so as to form a fin passageway, which is capable of receiving the bristled portion of a cytology brush. When the cytology brush is rotated, the bristles are agitated against the fins, thus dislodging cell samples. The cell samples are then dispersed within the solution and later retrieved. Disposed at the open end of the container is an insert. The insert has an alignment opening which acts to keep the trailing end of the cytology brush aligned with the leading bristled end of the brush, as it is being rotated within the fin passageway. While the cytology brush device is rotated within the cylindrical container, the container is securely held to a support surface with a Z-shaped clamp. The clamp is attached to a support surface with an adhesive.

Claims54

Dependent claims

A collection apparatus for containing a solution that is used for the retrieval of cell samples from bristles of a cytology brush device having a body with a leading end and a trailing end, the collection apparatus comprising:

(a) a container, adapted for containing a quantity of the solution, which has an open end and a closed end and wherein an interior portion of the container at the closed end is formed in a substantially conical shape;
(b) a cell removal means, disposed within the container at a point so as to be submerged within the solution contained therein and above the closed end so as to provide a space therebelow for collection of the cell samples, for providing at least one essentially horizontal and at least one essentially vertical agitation surface against which the bristles of the cytology brush device can be agitated so as to dislodge the cell samples present on the bristles and thus suspend the cell samples within the solution;
and
(c) a cap means for sealing the container in a fluid tight manner after the cytology brush device is removed from within the container.

1 more element

(a) a container, adapted for containing a quantity of the solution, which has an open end and a dosed end and wherein the closed end is formed in a substantially conical shape;
(b) a cell removal means, disposed within the container at a point so as to be submerged within the solution contained therein and above the closed end so as to provide a space therebelow for collection of the cell samples, for providing an agitation surface against which the bristles of the cytology brush device can be agitated so as to dislodge the cell samples present on the bristles and thus suspend the cell samples within the solution, the cell removal means comprising:
a plurality of fins affixed to an interior surface of the container, wherein each fin projects inwardly so that the fins meet in a spaced apart and opposed relationship and form a fin passageway therebetween, and wherein the plurality of fins are affixed to the interior surface of the container within the conical shaped closed end, and wherein each said fin has a top lateral edge, a side lateral edge, and a bottom end such that the side lateral edges of each fin act in combination to form the fin passageway between the inwardly projecting fins, and wherein the fin passageway is substantially uniform in cross section and has a central longitudinal axis that is concentric with the central longitudinal axis of the container, whereby the fin passageway is capable of receiving the leading end of the cytology brush device so that the bristles can be agitated against the top and/or the side lateral edge of each said fin as the cytology brush device is rotated;
and
(c) a cap means for sealing the container in a fluid tight manner when the cytology brush device is not present within the container.

2 more elements

(a) a container, adapted for containing a quantity of the solution, which has an open end and a closed end wherein an interior portion of the container at the closed end is formed in a substantially conical shape;
(b) a cell removal means, disposed within the container at a point so as to be submerged within the solution contained therein, for providing an agitation surface against which the bristles of the cytology brush device can be agitated so as to dislodge the cell samples present on the bristles and thus suspend the cell samples within the solution, the cell removal means comprising:
a plurality of fins affixed to an interior surface of the container, wherein each fin projects inwardly so that the fins meet in a spaced apart and opposed relationship and form a fin passageway therebetween, wherein the plurality of fins are affixed to the interior surface of the container, and wherein each said fin has a top lateral edge and a side lateral edge, such that the side lateral edges of each fin act in combination to form the fin passageway between the inwardly projecting fins, and wherein the fin passageway is substantially uniform in cross section and has a central longitudinal axis that is concentric with the central longitudinal axis of the container, whereby the fin passageway is capable of receiving the leading end of the cytology brush device so that the bristles can be agitated against the top and the side lateral edge of each said fin as the cytology brush device is rotated;
(c) an alignment means, disposed at the open end of the container, for providing an alignment aperture adapted to hold the body of the cytology brush device in alignment with the fin passageway as the leading end of the cytology brush device is advanced into the fin passageway, whereby the leading end of the cytology brush device tends to stay aligned with, and disposed within, the fin passageway as the cytology brush device is rotated and the bristles are agitated against the fins;
and
(d) a cap means for sealing the container in a fluid tight manner after the cytology brush device is removed from within the container.

3 more elements

(a) a container, adapted for containing a quantity of the solution, which has an open end and a closed end;
(b) a cell removal means, disposed within the container at a point so as to be submerged within the solution contained therein, for providing an agitation surface against which the bristles of the cytology brush device can be agitated so as to dislodge the cell samples present on the bristles and thus suspend the cell samples within the solution;
(c) a cap means for sealing the container in a fluid tight manner after the cytology brush device is removed from within the container;
and
(d) a holding means for releasably securing the container to a support surface so that the container is held in a stationary and rigid position while the cytology brush device is inserted and rotated therein and the bristles are agitated against the cell removal means, the holding means comprising:
(i) a base member having releasable attachment means for securement to the support surface;
and
(ii) first and second resilient gripping means mounted to the base member for receiving and holding the container, the first and the second resilient gripping means being together operative such that when the first and the second resilient gripping means are squeezed together the container can be slidably received by the first and the second resilient gripping means, and such that when the first and the second gripping means are released, the container is rigidly and non-slidably held by the first and the second gripping means and wherein the first gripping,means comprises a first planar surface having a first hole formed therein that has a diameter to as to be capable of slidably receiving said container, the first hole further having a first inner edge formed along the interior perimeter of said first hole, and wherein the second gripping means comprises a second planar surface having a second hole formed therein that has a diameter so as to be capable of slidably receiving said container, the second hole further having a second inner edge formed along the interior perimeter of said second hole, and wherein the first planar surface is formed at an angle with the second planar surface, the first and second planar surfaces further being resiliently moveable with respect to each other, whereby the first hole is aligned with the second hole by squeezing together the first planar surface and the second planar surface thus allowing the container to be slideably received within the first and second holes, the container thus being held in a friction tight manner by the first and the second said inner edges when the squeezing pressure is released.

5 more elements

(a) a cylindrical container, adapted for containing a quantity of the solution, which has an open end and a closed end and wherein an interior portion of the container at the closed end is formed in a substantially conical shape, where the outer periphery of the cylindrical container extends below the conical shape portion of the closed end so that the container may stand on a flat horizontal surface;
(b) a plurality of fins affixed to an interior surface of the conical shaped closed end of the container at a point so as to be submerged within the solution contained therein, each said fin having a top lateral edge that is slightly curved, and a bottom end that is spaced from the closed end of the container, and a side lateral edge, wherein each fin projects inwardly so that the side lateral edges meet in a spaced apart and opposed relationship so as to form a fin passageway therebetween, the fin passageway being substantially uniform in cross section and having a central longitudinal axis that is concentric with the central axis of the cylindrical container, whereby the fin passageway is capable of receiving the leading end of the cytology brush device so that the bristles can be agitated against the top and the side lateral edge of each said fin as the cytology brush device is rotated;
(c) a cylindrically shaped insert having a top and a bottom end where the outer perimeter of the top end is formed with an outwardly extending rim, the insert being slidingly received within the open end of the container and advanced therein to the point of the outwardly extending rim, the insert being held within the container in a friction-tight manner, the insert further having an alignment opening formed therein that has a shape that is circular with two laterally extending rectangular slits, the insert further having a first and a second guide surface formed on each side of the alignment opening, the alignment opening further being so sized and positioned so as to align the body of the cytology brush device with the fin passageway as the cytology brush device is rotated and the bristles are agitated against the fins;
(d) a threaded cap that is threadably attachable to the closed end of the container, the cap being capable of sealing the container in a fluid tight manner after the cytology brush device is removed from within the container;
and
(e) a resilient Z-shaped clamp for releasably securing the container to a support surface so that the container is held in a stationary and rigid position while the cytology brush device is inserted and rotated therein and the bristles are agitated against the cell removal means, the clamp comprising:
(i) a base member having a first adhesive affixed to the base member and a second and opposing adhesive affixed to the support surface, the first and the second adhesives being releasably attachable to one another;
(ii) a first planar surface, formed at an angle with the base member, the first planar surface having a first hole formed therein that has a diameter so as to be capable of slidably receiving said container, the first hole further having a first inner edge formed along the interior perimeter of said first hole;
and
(iii) a second planar surface, formed at an angle with the first planar surface, the second planar surface having, a second hole formed therein that has a diameter so as to be capable of slidably receiving said container, the second hole further having a second inner edge formed along the interior perimeter of said second hole;
whereby the first and second planar surfaces are resiliently moveable with respect to each other such that the first hole is aligned with the second hole by squeezing together the first planar surface and the second planar surface thus allowing the container to be slideably received within the first and second holes, the container then being held in a friction tight manner by the first and the second said inner edges when the squeezing pressure is released.

8 more elements

Dependencies are inferred from the claim text.

Cell collection apparatus

Life of this patent

Cell collection apparatus

Life of this patent

Abstract

Claims54