Industrial yeast comprising an integrated glucoamylase gene
AssigneeRobert R. Yocum
InventorsRobert R. Yocum, Robert S. Daves, Michael C. Chen
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Abstract
A vector having a gene for resistance to an antibiotic otherwise capable of killing a host yeast cell, the gene being transcribed from a yeast promoter sequence and the vector being capable of being integrated into a chromosome of the host yeast cell; and a diploid or greater ploidy yeast cell transformed by such a vector with heterologous DNA.
Claims56
An aneuploid, diploid or polyploid industrial Saccharomyces cell transformed with heterologous DNA, or a descendant of said cell, wherein said heterologous DNA is integrated into a chromosome of said Saccharomyces cell.
An aneuploid, diploid or polyploid industrial Saccharomyces cell transformed with a vector, or a descendant of said cell, said vector comprising:
- (a) a gene which confers resistance to an antibiotic otherwise capable of killing said Saccharomyces cell, said gene which confers resistance being transcribed from a yeast promoter sequence, and (b) heterologous DNA;
- wherein said heterologous DNA is integrated into a chromosome of said Saccharomyces cell.
An aneuploid, diploid or greater ploidy industrial Saccharomyces cell transformed with DNA encoding glucoamylase, or a descendant of said cell, wherein said Saccharomyces cell is capable of expressing enzymatically active glucoamylase from said DNA, and said DNA is integrated into a chromosome of said Saccharomyces cell.
An aneuploid or diploid or greater ploidy industrial Saccharomyces cell transformed with a vector, or a descendant of said transformed cell, said vector containing expressible DNA encoding glucoamylase, DNA encoding a selectable trait, and a DNA sequence homologous with a chromosomal sequence of said Saccharomyces cell;
- wherein said DNA encoding glucoamylase is integrated into a chromosome of said Saccharomyces cell.
Dependencies are inferred from the claim text.