Truncated human cholesterol 7.alpha.-hydroxylase, method of production and use thereof
AssigneeNortheastern Ohio Universities
InventorJohn Y. L. Chiang
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Abstract
A catalytically active, truncated human cholesterol 7.alpha.-hydroxylase (CYP7) is provided. The truncated human CYP7 can be made recombinantly and thus recovered in relatively large amounts. Vectors and host cells for recombinant expression are provided, as well as an antibody which specifically recognizes an epitope of catalytically active, truncated human CYP7. Also provided is a method for screening a compound for its effect on expression of non-truncated human CYP7, and a method for screening a compound for its effect on non-truncated human CYP7 enzyme activity.
Claims11
An expression vector comprising DNA encoding truncated human CYP7, wherein said expression vector when transfected into E. coli TOPP3, expresses catalytically active, truncated human CYP7.
A host cell having the characteristics of ATCC 69401.
Dependencies are inferred from the claim text.