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AssigneeOriental Yeast Co., Ltd.
InventorsHozumi Nishida, Masatsugu Nonobe, Tsuyoshi Fujita
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The present invention measures serum iron by liberating iron from transferrin under acid conditions, and contacting a test sample with a reaction system which utilizes at least aconitase, a reducing reagent, citric acid, isocitrate dehydrogenase and (thio)NAD(P). According to the present invention, an accurate and efficient measurement of iron in the serum is possible, even for minute levels of iron. In addition, the present invention assays unsaturated iron binding capacity by adding excess iron to a test sample to bind iron to transferrin, binding the remaining unbound iron to aconitase, and measuring the activated aconitase. According to the present invention, a quick and easy assay of unsaturated iron binding capacity of body fluids, etc. is possible without the need for expert skill.
A method for measuring the amount of iron in a fluid sample comprising liberating iron in said fluid sample, adding to the sample an effective amount of aconitase for binding the liberated iron to the aconitase, and measuring resulting aconitase activity.
A method for measuring the amount of iron in a serum sample comprising liberating iron in said serum sample, adding to the sample an effective amount of aconitase for binding the liberated iron to the aconitase, and measuring resulting aconitase activity.
An enzymatic assay method for serum iron, characterized by including a step of liberation of iron from transferrin in serum, a step of binding the liberated iron to aconitase, and a step of measuring aconitase activity.
An enzymatic assay method for serum iron, characterized by including a step of liberation of iron from transferrin, to which iron is bound in blood, and a step of measuring aconitase activity while binding the liberated iron to aconitase.
A reagent for enzymatic measurement of serum iron consisting essentially of apo-aconitase, a reducing agent, citric acid, isocitrate dehydrogenase, and a compound selected from the group consisting of nicotinamide adenine dinucleotides and nicotinamide adenine dinucleotide phosphates.
A reagent for enzymatic measurement of iron comprising:
A method for assaying unsaturated iron binding capacity in a sample comprising:
A method for assaying unsaturated iron binding capacity in a sample comprising:
Dependencies are inferred from the claim text.