Glycosyl-phosphatidylinositol-specific phospholipase D

An isolated gene which encodes the protein glycosyl-phosphatidylinositol-specific phospholipase D and has the nucleotide sequence of FIG. 9.

A synthetic polynucleotide molecule expressing a protein which is connected to a glycosyl-phosphatidylinositol anchor molecule, which polynucleotide molecule comprises both a first nucleotide sequence encoding glycosyl-phosphatidylinositol-specific phospholipase D, and a second nucleotide sequence encoding said protein to be expressed, said second nucleotide sequence being directly linked at its 3' end to a third nucleotide sequence encoding a C-terminal signal peptide obtained from a GPI anchored protein, which C terminal signal peptide directs the attachment of a glycosyl-phosphatidyl inositol anchor molecule to the protein to be expressed.

A vector designed for expressing protein which expresses a protein which is connected to a glycosyl-phosphatidylinositol anchor molecule, which vector contains both a first nucleotide sequence encoding glycosyl-phosphatidylinositol-specific phospholipase D, and a second nucleotide sequence encoding said protein to be expressed said second nucleotide sequence being directly linked at its 3' end to a third nucleotide sequence encoding a C-terminal signal peptide which directs the attachment of a glycosyl-phosphatidyl inositol anchor molecule to the protein to be expressed, such sequences suitably located in the vector so as to express a protein.

A transformed host cell which produces glycosyl-phosphatidylinositol anchor molecules, which host cell contains a vector designed for expressing protein-inserted therein comprising a first nucleotide sequence encoding glycosyl-phosphatidyl inositol-specific phospholipase D, and a second nucleotide sequence encoding a protein to be expressed, said second nucleotide sequence being directly linked at its 3' end to a third nucleotide sequence encoding a C-terminal signal peptide which directs the attachment of a glycosyl-phosphatidylinositol anchor molecule to the protein to be expressed, such sequences suitably located in the vector so as to express a protein.

A transformed host cell which produces glycosyl-phosphatidylinositol anchor molecules, which host cell contains inserted therein both a first vector designed for expressing protein comprising a first nucleotide sequence encoding glycosyl-phosphatidylinositol-specific phospholipase D, and a second vector designed for expressing protein comprising a second nucleotide sequence encoding a protein to be expressed, said second nucleotide sequence being directly linked at its 3' end to a third nucleotide sequence encoding a C-terminal signal peptide which directs the attachment of a glycosyl-phosphatidylinositol anchor molecule to the protein to be expressed, such sequences suitably located in the vectors so as to express proteins.