Method of inactivation of viral and bacterial blood contaminants
AssigneeCryopharm Corporation
InventorsMatthew S. Platz, Raymond P. Goodrich, Jr., Nagendar Yerram
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Abstract
A method is provided for inactivating viral and/or bacterial contamination in blood cellular matter, such as erythrocytes and platelets, or protein fractions. The cells or protein fractions are mixed with chemical sensitizers and irradiated with, for example, UV, visible, gamma or X-ray radiation. In particular, quaternary ammonium or phosphonium substituted, halo-psoralen compounds are described as being useful.
Claims41
A process for reducing vital, and/or bacterial contaminants in a composition comprising blood, a blood component, cell cultures or a component of a cell culture, comprising the steps:
- mixing said composition in a liquid state with a chemical radiation sensitizer capable of targeting said vital, and/or bacterial contaminants, wherein said sensitizer is a compound of the formula:
- ##STR80## wherein u is an integer from 1 to 6;
- X is an anionic counterion;
- R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, and R.sub.6 are independently halo;
- H;
- linear or branched alkyl of 1-10 carbon atoms;
- linear or branched alkoxy of 1-10 carbon atoms;
- (CH.sub.2).sub.m O(CH.sub.2).sub.p Z.sup..sym. R', R", R"' or --O(CH.sub.2).sub.n Z.sup..sym. R', R", R"' wherein n, m and p are independently integers from 1 to 10 and R', R", R"' are independently H or linear or branched alkyl of 1 to 10 carbon atoms;
- at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, and R.sub.6 is halo;
- and at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, and R.sub.6 is (CH.sub.2).sub.m O(CH.sub.2).sub.p Z.sup. .sym. R', R", R"' or --O(CH.sub.2).sub.n Z.sup..sym. R', R"';
- Z is N or P;
- and
- exposing said composition and sensitizer to electromagnetic radiation of sufficient wavelength and intensity for a period of time sufficient to activate said sensitizer whereby the activation of said sensitizer reduces said contamination in said composition.
Dependencies are inferred from the claim text.