Process for extracting minor fatty compounds from a substance of biological origin
AssigneeRoquette Freres
InventorsLeon Mentink, Michel Serpelloni
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Abstract
A method for extracting minor fatty compounds, particularly steroids, from biological matter by means of cyclodextrin. According to the method, the cyclodextrin is stirred into said biological matter in a water-containing reaction medium to allow the formation of inclusion complexes between the cyclodextrin and the minor fatty compounds, whereafter said complexes are separated from said matter, the amount of water present in the reaction medium being lower than 100 % wt. in relation to the matter to be treated. The temperature of the reaction medium and the amount of water present therein are such that the cyclodextrin is partially undissolved.
Claims20
A process for extracting minor fatty compounds selected from the group consisting of steroids, fatty alcohols, fat soluble vitamins, pigments, hydrocarbons and flavouring compounds from a substance of biological origin containing fatty substances, comprising the steps of:
- selecting a substance of biological origin containing said minor fatty compounds and fatty substances;
- selecting a cyclodextrin in pulverulent form capable of forming inclusion complexes with said minor fatty compounds, from the group consisting of substituted and unsubstituted alpha, beta and gamma cyclodextrin;
- providing an amount of said cyclodextrin of from 0.5 to 15% relative to the dry matter of the substance of biological origin to be treated;
- contacting the amount of cyclodextrin with the substance of biological origin in an aqueous reaction medium at a temperature about 10.degree. C. to 25.degree. C. and maintaining the water content of the reaction medium so as to obtain a saturation level of cyclodextrin in water equal to or greater than 1.67 at the reaction temperature and so that the cyclodextrin is in a partially undissolved form;
- mixing during 10 to 120 minutes the cyclodextrin with the substance of biological origin so as to obtain a pasty mixture and to allow the formation of the said inclusion complexes;
- separating, the said inclusion complexes formed during the contacting step from the said substance of biological origin by centrifugation, by decantation, or by heating the mixture obtained to a temperature at which not more than 1% of the fatty substances present in the substance of biological origin are in a solid or crystalline state;
- and
- recovering the substance of biological origin with a reduced content of minor fatty compounds.
Dependencies are inferred from the claim text.