Method and system for determining bioactive substances
AssigneeNational Research Council of Canada
InventorsJohn H. T. Luong, Keith B. Male, Maurice V. Cattaneo
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Abstract
An apparatus and method for measuring glutamine and glucose in certain cell cultures and body fluids in the presence of interfering endogenous compounds e.g. ascorbic acid, uric acid or glutamic acid. Prior to enzymatic degradation and amperometric detection of the products or elements resulting from the degradation, the interfering substances are retained by an anion exchanger disposed upstream of the enzymatic degradation site. The conditions of the method are controlled to utilize the difference of isoelectric points of the measured biosubstance and of the interfering compounds respectively.
Claims7
A detection system for measuring glutamine in a liquid sample using enzymatic degradation of glutamine and amperometric detection of the resulting product or element, in the presence of compounds interfering with the measurement, the system comprising in combination:
- a) an ion exchange means capable of at least partly removing from the sample passed therethrough the interfering compounds while leaving the measured glutamine therein by virtue of a difference in their respective electric charges,
- b) immobilized enzymes glutaminase and glutamate oxidase for the enzymatic degradation of the measured glutamine, the enzymes being associated with the ion exchange means downstream thereof, and
- c) a sensor capable of sensing a product or element resulting from the enzymatic degradation of said glutamine to produce a signal indicative of the concentration of glutamine in the sample.
A method of measuring glutamine in a liquid sample containing substances normally interfering with the measurement of said glutamine by enzymatic degradation of said glutamine and amperometric detection of a product or element resulting from the enzymatic degradation, said method comprising:
- a) passing the sample through an ion exchange means at a pH selected to impart a different electric charge on the particles of the interfering substances in said sample compared to the electric charge on the particles of the glutamine, to effect at least a partial retention of the interfering substances by said ion exchange means, then
- b) subjecting said sample to enzymatic degradation to form an enzymatic reaction product, and
- c) sensing the concentration of said reaction product or of another compound or element consumed or liberated in the formation of said product, said concentration being indicative of the concentation of glutamine in the sample.
Dependencies are inferred from the claim text.