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AssigneeNippon Kayaku Kabushiki Kaisha
InventorsMasahiko Yabuuchi, Hiroshi Akanuma, Minoru Masuda, Kazuo Katoh, Tsuneo Nakamura, Shigeru Tajima, Masashi Hashiba, Hiroshi Hayami, Tomoko Takezawa, Masachika Hirayama
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A present invention relates to a method for quantitatively determining sugar-alcohols characterized by passing test samples containing sugar-alcohols, proteins, and saccharides through a column filled with basic anion-exchange resins which have a protein-removing ability and a saccharide-removing ability, and then quantitaing the sugar-alcohols in the effluent out of the column, a column filled with said resins and an aqueous solution of boric acid, and a kit. Sugar-alcohols such as 1,5-anhydroglucitol and the like have been calling attention as markers for diabetes mellitus recently.
A method for quantitatively determining one or more sugar-alcohol comprising passing test samples containing sugar-alcohols, proteins, and saccharides through a column packed with a basic anion-exchange resin or resins which are hydrophilic polymer resins having a quaternary ammonium group and no aromatic ring, are in the ionic OH.sup.- or borate form, and which have a protein removing ability and a saccharide-removing ability, and then quantitating one or more sugar-alcohol in the effluent out of the column.
A method for quantitatively determining 1,5-anhydroglucitol which comprises passing test samples containing sugar-alcohols, proteins and saccharides through a column packed with strongly basic anion-exchange resin or resins which are in the ionic OH.sup.- or borate form and are selected from the group consisting of polyvinylalcohol hydrophilic gel filtrating resin or resins and polymethacrylate hydrophilic gel filtrating resin or resins having tri-lower (C.sub.1 -C.sub.4) alkyl ammonium groups introduced as exchanging groups, and quantitating 1,5-anhydroglucitol in the effluent.
A kit comprising a column packed with basic anion-exchange resin or resins which are hydrophilic polymer resin or resins having a quaternary ammonium group and no aromatic ring and which have a protein-removing ability and a saccharide-removing ability and with an aqueous solution of boric acid, or reagents for quantitating one or more sugar-alcohol and one or more sugar-alcohol for preparing calibration lines.
A method for determining 1,5-anhydroglucitol in blood comprising injecting sequentially a number of test samples containing sugar-alcohols, proteins and saccharides into a system comprising an interfering substance-removing column through which a liquid is flowing, said column packed with strongly basic anion-exchange resin or resins comprising hydrophilic high molecular weight carrier or carriers having quaternary ammonium groups introduced, but not having any aromatic ring and which are in the ionic OH.sup.- or borate form, an injector, and a biosensor, and quantitating 1,5-anhyroglucitol in the effluent.
Dependencies are inferred from the claim text.