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AssigneeETC Elektrophorese-Tecknik Westermeier & Schickle GmbH
InventorHanspeter Schickle
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For high-resolution two-dimensional electrophoresis comprising the steps of carrying out, on a gel base, at first a first separation process in a first direction and then a second separation process in a direction vertical to the first direction by a technique different from that of the first separation, for example by molecular screening, it is proposed to use as a basis a dry gel consisting of a single piece and extending in the two dimensions and to hydrate the dry gel selectively in a strip-like area for carrying out the isoelectric separation in the first dimension, while the remaining gel area retains its dry gel configuration, to then perform the separation in the first dimension in the selectively hydrated area, and to hydrate thereafter the remaining electrophoresis area of the gel for carrying out the second separation process.
A method for high-resolution two-dimensional electrophoresis comprising the steps of carrying out at first, on a gel base, a first separation process in a first direction by isoelectric focussing or any other electrophoretic separation technique, and performing thereafter a second separation process in a direction vertical to the first direction by a technique different from that of the first separation,
A device for high-resolution two-dimensional electrophoresis where, using a gel base, at first a first separation process is carried out in a first direction by isoelectric focussing and then, using the gel base, the second separation is performed in a direction vertical to the first direction, wherein a single dry gel is provided as a basis and means are provided for selectively hydrating at first the dry gel in a partial area for carrying out the first separation so that a strip-like area of the dry gel is soaked and expanded uniformly with the mixtures to be used for carrying out the first separation process.
Dependencies are inferred from the claim text.