Back to results
US 5,405,772 AGranted
Medium for long-term proliferation and development of cells
AssigneeAmgen Inc.
InventorIan L. O. Ponting
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeAmgen Inc.
InventorIan L. O. Ponting
Estimating the term…
A serum-free or serum-depleted medium for the short- and long-term proliferation and development of cells, particularly hematopoietic cells and bone marrow stromal cells, the medium comprising cell proliferation and development effective amounts of: a standard culture medium such as Iscove's modified Dulbecco's medium; serum albumin; transferrin; a source of lipids and fatty acids; cholesterol; a reducing agent; pyruvate; a glucocorticoid (when the cells to be cultured are hematopoietic cells); nucleosides for synthesis of DNA and RNA; growth factors that stimulate the proliferation and development of stromal cells and cells from a variety of tissues or organs, such as epidermal growth factor, fibroblast growth factor, platelet derived growth factor, and insulin; and extracellular matrix materials, such as collagen, fibronectin, and laminin.
A medium for the long-term proliferation and development of cells, which comprises the following ingredients in the indicated amounts:
Dependencies are inferred from the claim text.