Synthesis of human procollagens and collagens in recombinant DNA systems
AssigneeThomas Jefferson University
InventorsDarwin J. Prockop, Leena Ala-Kokko, Andrzej Fertala, Aleksander Sieron, Kari I. Kivirikko, Amy Geddis
Life of this patent
Estimating the term…
Abstract
The invention is stably transfected cells, substantially all of which contain at least one human collagen gene and express fibrillar collagen molecules derived using methods for synthesizing collagen and collagen fibrils in said cell lines, and methods for treatment of disorders in humans using said collagen derived from said stable cell lines.
Claims25
Human tumor cells substantially all of which comprise at least one transfected human pro.alpha.1(I) procollagen gene or human pro.alpha.1(I) collagen gene, and which express human procollagen or human collagen molecules having at least one chain derived from said transfected human procollagen or human collagen gene or genes, wherein any chains derived from genes other than said transfected human genes are derived from expression of endogenous genes.
A method for synthesizing procollagen or collagen in human tumor cells comprising the steps of:
- a) transfecting at least one human pro.alpha.1(I) procollagen gene or human pro.alpha.1(I) collagen gene into said human tumor cells,
- b) culturing said human tumor cells under conditions such that said transfected human procollagen gene or transfected human collagen gene are expressed;
- c) selecting transfected humor tumor cells that comprise at least one transfected human pro.alpha.1(I) procollagen gene or human pro.alpha.1(I) collagen gene, and which express human procollagen or human collagen molecules having at least one chain derived from said transfected human procollagen or human collagen gene or genes, wherein any chains derived from genes other than said transfected human genes are derived from expression of endogenous genes.
Human tumor cells substantially all of which comprise at least one transfected gene that encodes human pro.alpha.1(I) procollagen or human pro.alpha.1(I) collagen and at least one transfected gene that encodes human pro.alpha.2(I) procollagen or human pro.alpha.2(I) collagen, wherein said human tumor cells express human type I procollagen [pro.alpha.1(I).sub.2 ]pro.alpha.2(I) molecules.
Dependencies are inferred from the claim text.