Pulsed oriented electrophoresis

Abstract

Disclosed herein is a method of electrophoretically separating particles by the application to pulsed field electrophoresis of a pulsing routine in which the pulses are modulated in such a way as to define the effective field angle of the particles undergoing separation as determined by the vector sum of the pulses. In one embodiment of the invention, pulse times are modulated to define the orientation of the particles undergoing separation. In a second embodiment, the intensities of the pulses are modulated to define the orientation of the particles. The method of the invention allows the simple and dynamic determination of effective field angle by pulsing routine rather than by placement of an electrode array. In addition, the invention makes it possible for the first time to translate particles undergoing separation incrementally through a gel matrix.

Dependencies are inferred from the claim text.