Capillary column method translation
AssigneeHewlett-Packard Company
InventorsWarren D. Snyder, Leonid M. Blumberg
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Abstract
The invention is a method for modifying a known chromatographic method without changing peak elution order and with little or no change in resolution. Column and operational parameters of a known method are systematically translated to ensure that a new chromatographic method having possibly different column and operational parameters has a chromatographic output with similar separation.
Claims5
A computer-based method of controlling a gas chromatograph running system software to produce a new chromatographic output by automatically reconfiguring existing operating parameters of the gas chromatograph in response to the input of new column parameters and a new column, said new chromatographic output being substantially equivalent to a known chromatographic output of a known chromatographic method having both existing column parameters and existing operating parameters, comprising the steps of:
- storing existing column parameters and existing operating parameters of the known chromatographic method on the system software;
- and
- storing known translation equations on the system software, said translation equations being based on the ratio of new column parameters to existing column parameters;
- and
- inputting new column parameters corresponding to said new column into the system software;
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- calculating the ratios between new column parameters and existing column parameters;
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- calculating new operating parameters based on the ratios between new column parameters and existing column parameters;
- and
- automatically reconfiguring the existing operating parameters of said gas chromatograph with said new operating parameters;
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- injecting a sample into said gas chromatograph for chromatographic separation into components, said components being outputted serially from said gas chromatograph;
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- generating an electrical signal corresponding to the serially outputted components;
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- displaying said electrical signal, said signal being the new chromatographic output of said gas chromatograph with said new column and new operating parameters, said new chromatographic output being substantially the same as said existing chromatographic output of said gas chromatograph with said existing column parameters and existing operating parameters.
Dependencies are inferred from the claim text.