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AssigneeBioControl Systems, Inc.
InventorsN. Robert Ward, Jr., John P. DesRosier, Elliott D. Marshall, III, Judith Ford, Nancy J. S. Mallinak
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There is disclosed an improved method for testing for the presence of a particular microorganism or group of microorganisms characterized by a particular enzyme. The method uses a dye-forming substrate throughout a polymer matrix or on the surface of a solid support member that forms a colored precipitate when cleaved by the enzyme. The precipitate is concentrated to the polymer matrix and/or solid support member to create a visible reaction product, wherein the amount of dye-forming substrate needed is independent of the sample size. The present invention further comprises an enzyme indicator device comprising a dye-forming substrate throughout a polymer system or on the surface of a solid support member.
A method for assaying for an enzyme associated with a particular species of microorganism or a group of microorganisms in a sample, wherein the particular species of microorganism is E. coli and the group of microorganisms is the coliform group of bacteria comprising:
An enzyme indicator device for assaying for the presence of either the species E. coli or the coliform group of bacteria consisting essentially of one or more dye-forming substrates adhered to a surface of a solid support wherein the one or more substrates are cleaved by an enzyme associated with E. coli or the coliform group of bacteria to form an insoluble colored precipitate located at the reaction surface, and wherein the dye-forming substrate is a substituted indigo derivative of the formula:
An enzyme indicator device for assaying for the presence of either the coliform group of bacteria or for the species E. coli consisting essentially of a first dye-forming substrate and a second dye-forming substrate, each of the formula:
Dependencies are inferred from the claim text.