Antibiotic AB-041 derived from Streptomyces sp. NCIMB 40428, herbicidal compositions, and methods of use
AssigneeMinistero Dell `Universita` E Della Ricerca Scientifica E Tecnologica
InventorsNunzio Andriollo, Alessandro Scacchi, Giorgio E. Borgonovi, Giorgio Cassani, Silvia Spera, Gianfranco Guglielmetti, Giorgio Pirali, Giovanni Confalonieri
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Abstract
The antibiotic AB-041 is described, obtained by controlled aerobic cultivation of Streptomyces sp. NCIMB 40428 in an aqueous culture medium. The antibiotic AB-041 exhibits biological activity, and in particular herbicidal activity.
Claims19
An AB-041 antibiotic, produced by Streptomyces sp. NCIMB 40428 or a mutant equivalent thereof during controlled aerobic cultivation in an aqueous culture medium containing carbon, nitrogen and inorganic salt sources;
- said antibiotic having:
- an ultraviolet absorption maximum at 278 nm,
- infrared absorption maxima (cm.sup.-1) at 3395, 1663, 1594, 1449, 1403, 1338, 1307, 1285, 1246, 1225, 1159, 1102, 1074, 1037, 1017, 960, 942, 917, 888 and 741, and
- an empirical formula of C.sub.16 H.sub.25 N.sub.5 O.sub.9 S.
An antibiotic AB-041, having the following properties:
- (a) good solubility in water, in dimethylsulphoxide and dimethylsulphoxide/water mixtures, but practically insoluble in ethyl ether and hexane;
- (b) by elemental analysis, contains carbon, hydrogen, nitrogen, oxygen and sulphur;
- (c) a molecular weight of 463 g/mol;
- (d) an ultraviolet absorption maximum at 278 nm;
- (e) infrared absorption maxima at 3395, 1663, 1594, 1449, 1403, 1338, 1307, 1285, 1246, 1225, 1159, 1102, 1074, 1037, 1017, 960, 942, 917, 888 and 741 cm.sup.-1 ;
- (f) an .sup.1 HNMR spectrum having principal peaks, relative to TMS, at (ppm):
- 8.26 (s, 1H), 4.55 (t, 1H), 4.43 (d, 1H), 4.26 (d, 1H), 4.04 (m, 1H), 3.67 (m, 3H), 3.36 (t, 1H), 3.26 (t, 1H), 3.09 (m, 1H), 2.71 (dd, 1H), 2.19 (d, 1H) and 1.26 (d, 3H);
- (g) a .sup.13 CNMR spectrum having principal peaks, relative to TMS, at (ppm):
- 181.3 (s), 174.7 (s), 165.8 (s), 158.3 (s), 137.8 (s), 107.8 (s), 105.3 (s, very weak), 73.1 (d), 66.8 (t), 62.0 (t), 51.1 (d), 47.3 (t), 43.6 (d), 43.6 t), 42.0 (d) and 18.5 (q);
- (h) a positive colorimetric reaction with ninhydrin in acetone (0.2% w/v) and with pinacryptol yellow in water (0.07% w/v), and a negative colorimetric reaction with a diazotization reagent and coupling with .alpha.-naphthol;
- and
- (i) a retention factor (Rf) of 0.35 on a cellulose F chromatography plate with 91:9 acetonitrile/water (v/v) as eluent.
The micro-organism Streptomyces sp. NCIMB 40428.
Dependencies are inferred from the claim text.