Biologically pure culture of bacillus ATCC 55294 that produces an endoglycanase
AssigneesShin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
InventorsLinda P. Thorne, Richard W. Armentrout, Thomas J. Pollock, Marcia J. Mikolajczak
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Abstract
An isolated endogly canase having activity against polysaccharides having the subunit backbone structure: ##STR1## wherein Glc is glucose, GlcA is glucuronic acid, Rha is rhamnose, Man is mannose, X may be Rha or Man, and wherein the reducing end of the polymer is toward the X residue of the backbone is disclosed. A method for producing the compound by culturing Bacillus ATCC 55294, the method for selecting Bacillus ATCC 55294 and method for using the endoglycanase are also disclosed.
Claims7
A biologically pure culture of Bacillus ATCC 55294 which produces an endoglycanase having endolytic activity against polysaccharides having the subunit backbone structure;
- ##STR18## wherein Glc is glucose, GlcA is glucuronic acid, Rha is rhamnose, Man is mannose, X may be Rha or Man, and wherein the reducing end of the polymer is toward the X residue of the backbone.
A biologically pure culture of Bacillus ATCC 55294 which produces an endoglycanase having endolytic activity against polyseccharides having the subunit backbone structure:
- ##STR19## wherein Glc is glucose, ClcA is glucuronic acid, Rha is rhamnose, Man is mannose, X may be Rha or Man, and wherein the reducing end of the polymer is toward the X residue of the backbone, said Bacillus ATCC 55294 being obtained by:
- a) subjecting a naturally occurring collection of bacterial straining to growth conditions in a medium containing a polysaccharide having the subunit backbone structure;
- ##STR20## wherein the reducing end of the polymer is toward the Rha residue of the backbone, as the predominate carbon source;
- b) selecting a colony of cells which grow under the conditions of step a);
- c) subjecting the selected colony from step b) to plate growth conditions in a gel medium containing the polysaccharide having structure I as the predominant carbon source;
- and
- d) selecting a strain from step c) which causes pitting of the gel.
Dependencies are inferred from the claim text.