Method of preparing Limulus amoebocyte lysate
AssigneeSeikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)
InventorsShigenori Tanaka, Jun Aketagawa, Yuko Shibata
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Abstract
Disclosed is a method of preparing limulus amoebocyte lysate substantially free from factor G which comprises bringing limulus amoebocyte lysate into contact with an insoluble carrier on which a (1.fwdarw.3)-.beta.-D-glucoside structural portion represented by the following formula [I] produced by depolymerizing and/or fractionating a carbohydrate chain is immobilized: ##STR1## wherein n represents an integer of 2 to 370.
Claims5
A method of preparing limulus amoebocyte lysate substantially free from factor G which comprises bringing limulus amoebocyte lysate into contact with an insoluble carrier on which a (1.fwdarw.3)-.beta.-D-glucoside structural portion represented by the following formula (I) produced by depolymerizing and/or fractionating a carbohydrate chain is immobilized by a method which comprises epoxy-activating an insoluble carrier with epichlorohydrin or bisoxiranes, reacting the resulting epoxy-activated insoluble carrier with hydrazine hydrate or a dihydrazide compound to obtain a hydrazine derivative or a dihydrazide derivative and bringing the (1.fwdarw.3)-.beta.-D-glucoside into contact with the hydrazine or dihydrazide derivative:
- ##STR10## wherein n represents an integer of 2 to 370.
Dependencies are inferred from the claim text.