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US 5,401,646 AGranted
Process for production of bishomo-gamma-linolenic acid and eicosapentaenoic acid
AssigneeSuntory Limited
InventorsYoshifumi Shinmen, Hideaki Yamada, Sakayu Shimizu
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Abstract
A process for the production of a fatty acid selected from the group consisting of bishomo-.gamma.-linolenic acid and eicosapentaenoic acid comprising the steps of culturing a microorganism belonging to the genus Mortierella and capable of producing at least one of the fatty acids to obtain a cultured product, and recovering at least one of the fatty acids; and a process for the production of lipid containing such fatty acids.
Claims8
A process for producing bishomo-.gamma.-linolenic acid, comprising the steps of:
- culturing in a nutrient medium containing an additive selected from the group consisting of a hydrocarbon, a fatty acid, a salt of a fatty acid, and a lipid, a microorganism selected from the group consisting of Mortierella elongata FERM BP-1239, Mortierella exigua IFO 8571, Mortierella hygrophila IFO 5941, and Mortierella elongata IFO 8570, to produce bishomo-.gamma.-linolenic acid;
- and
- recovering the bishomo-.gamma.-linolenic acid.
A process for producing bishomo-.gamma.-linolenic acid, comprising the steps of:
- culturing in a nutrient medium a microorganism selected from the group consisting of Mortierella elongata FERM BP-1239, Mortierella exigua IFO 8571, Mortierella hygrophila IFO 5941, and Mortierella elongata IFO 8570;
- adding to said medium during culturing an additive selected from the group consisting of a fatty acid, a salt of fatty acid, and a lipid, to produce bishomo-.gamma.-linolenic acid;
- and
- recovering the bishomo-.gamma.-linolenic acid.
1 more element
A process for producing a lipid containing bishomo-.gamma.-linolenic acid, comprising the steps of:
- culturing in a nutrient medium containing an additive selected from the group consisting of a hydrocarbon, a fatty acid, a salt of a fatty acid, and a lipid, a microorganism selected from the group consisting of Mortierella elongata FERM BP-1239, Mortierella exigua IFO 8571, Mortierella hygrophila IFO 5941, and Mortierella elongata IFO 8570, to obtain a cultured broth;
- separating said cultured broth to obtain microbial cells and a supernatant;
- extracting said microbial cells with an extraction agent to obtain an extract;
- and
- eliminating the extraction agent from the extract to obtain the lipid containing bishomo-.gamma.-linolenic acid.
2 more elements
A process for producing a lipid containing bishomo-.gamma.-linolenic acid, comprising the steps of:
- culturing in a nutrient medium a microorganism selected from the group consisting of Mortierella elongata FERM BP-1239, Mortierella exigua IFO 8571, Mortierella hygrophila IFO 5941, and Mortierella elongata IFO 8570;
- adding to said medium during culturing an additive selected from the group consisting of a fatty acid, a salt of a fatty acid, and a lipid, to obtain a cultured broth;
- separating said cultured broth to obtain microbial cells and a supernatant;
- extracting said microbial cells with an extraction agent to obtain an extract;
- and
- eliminating the extraction agent from the extract to obtain the lipid containing bishomo-.gamma.-linolenic acid.
3 more elements
A process for producing eicosapentaenoic acid, comprising the steps of:
- culturing in a nutrient medium containing an additive selected from the group consisting of a hydrocarbon, a fatty acid, a salt of a fatty acid, and a lipid, a microorganism selected from the group consisting of Mortierella elongata FERM BP-1239, Mortierella exigua IFO 8571, Mortierella hygrophila IFO 5941, and Mortierella elongata IFO 8570;
- and
- recovering eicosapentaenoic acid, wherein the culturing is carried out at a temperature between 10.degree. C. and 20.degree. C. for a sufficient period of time to produce eicosapentaenoic acid.
A process for producing eicosapentaenoic acid, comprising the steps of:
- culturing in a nutrient medium a microorganism selected from the group consisting of Mortierella elongata FERM BP-1239, Mortierella exigua IFO 8571, Mortierella hygrophila IFO 5941, and Mortierella elongata IFO 8570;
- adding to said medium during culturing an additive selected from the group consisting of a fatty acid, a salt of a fatty acid, and a lipid, to produce eicosapentaenoic acid;
- and
- recovering the eicosapentaenoic acid, wherein the culturing is carried out at a temperature between 10.degree. C. and 20.degree. C. for a sufficient period of time to produce eicosapentaenoic acid.
1 more element
A process for producing a lipid containing eicosapentaenoic acid, comprising the steps of:
- culturing in a nutrient medium containing an additive selected from the group consisting of a hydrocarbon, a fatty acid, a salt of a fatty acid, and a lipid, a microorganism selected from the group consisting of Mortierella elongata FERM BP-1239, Mortierella exigua IFO 8571, Mortierella hygrophila IFO 5941, and Mortierella elongata IFO 8570, to obtain a cultured broth, wherein the culturing is carried out at a temperature between 10.degree. C. and 20.degree. C. for a sufficient period of time to produce a lipid containing eicosapentaenoic acid;
- separating said cultured broth to obtain microbial cells and a supernatant;
- extracting said microbial cells with an extraction agent to obtain an extract;
- and
- eliminating the extraction agent from the extract to recover the lipid containing eicosapentaenoic acid.
2 more elements
A process for producing a lipid containing eicosapentaenoic acid, comprising the steps of:
- culturing in a nutrient medium containing a microorganism selected from the group consisting of Mortierella elongata FERM BP-1239, Mortierella exigua IFO 8571, Mortierella hygrophila IFO 5941, and Mortierella elongata IFO 8570;
- adding to said medium during culturing an additive selected from the group consisting of a fatty acid, a salt of a fatty acid, and a lipid to obtain a cultured broth, wherein the culturing is carried out at a temperature between 10.degree. C. and 20.degree. C. for a sufficient period of time to produce a lipid containing eicosapentaenoic acid;
- separating said cultured broth to obtain microbial cells and a supernatant;
- extracting said microbial cells with an extraction agent to obtain an extract;
- and
- eliminating the extraction agent from the extract to recover the lipid containing the eicosapentaenoic acid.
3 more elements
Dependencies are inferred from the claim text.