Detection and quantification of neu related proteins in the biological fluids of humans
AssigneeOncogene Science, Inc.
InventorsWalter P. Carney, Sara J. McKenzie
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Abstract
This invention relates to a substantially purified p100 which is a human neu related protein having a molecular weight in the range from about 97,000 daltons to about 115,000 daltons which corresponds substantially to the extracellular domain of the human neu gene product, said protein being detectable in a biological fluid. In another embodiment this invention relates to assays for detecting this protein. Finally, this invention also concerns monoclonal antibodies which are capable of binding to p100.
Claims11
An immunoassay for detecting the presence of p100 in a sample of human plasma or serum which comprises:
- (a) reacting the sample of human plasma or serum with a first antibody which specifically binds an epitope of the extracellular domain of human neu gene encoded product;
- (b) reacting the product of step (a) with a detectably-labeled second antibody which specifically binds the extracellular domain of human neu gene encoded product at an epitope different from the epitope bound by the first antibody;
- and
- (c) detecting the product of step (b), and thereby detecting the presence of the p100 in the sample.
Dependencies are inferred from the claim text.