Polypeptides derived from the human immunodeficiency virus endonuclease protein
AssigneeOrtho Diagnostic Systems Inc.
InventorFrancesca Chiodi
Life of this patent
Estimating the term…
Abstract
A polypeptide linked to a solid matrix useful as a solid support of a diagnostic kit is disclosed. The polypeptide has the formula: X-Glu-Thr-Gly-Gln-Glu-Thr-Ala-Tyr-Phe-B-Leu-Lys-Leu-Ala-Gly-Arg-Trp-Pro-Va l-Lys-Z, wherein B is Ile or Leu, X is a chain of from 1 to 20 amino acid residues or an amino-terminal group and Z is a chain of from 1 to 20 amino acid residues or a carboxy-terminal group. The polypeptide immunologically mimics HIV endonuclease.
Claims7
A solid support comprising a solid matrix having an affixed polypeptide, said affixed polypeptide having the formula (SEQ ID NO:1) ##STR8## wherein:
- Xaa is Leu or Ile,
- X is a chain of 1 to 10 amino acid residues selected from the group consisting of
- Gly-Tyr-Ile-Glu-Ala-Glu-Val-Ile-Pro-Ala (SEQ ID NO:3);
- Gly-Tyr-Ile-Glu-Ala-Glu-Val-Ile-Pro (SEQ ID NO:4);
- Gly-Tyr-Ile-Glu-Ala-Glu-Val-Ile (SEQ ID NO:5);
- Gly-Tyr-Ile-Glu-Ala-Glu-Val (SEQ ID NO:6);
- Gly-Tyr-Ile-Glu-Ala-Glu (SEQ ID NO:7);
- Gly-Tyr-Ile-Glu-Ala (SEQ ID NO:8);
- Gly-Tyr-Ile-Glu (SEQ ID NO:9);
- Gly-Tyr-Ile;
- Gly-Tyr;
- and
- Gly;
- or an amino-terminal NH.sub.2 group;
- and
- Z is a chain of 1 to 10 amino acid residues selected from the group consisting of
- Thr-Ile-His-Thr-Asp-Asn-Gly-Ser-Asn-Phe (SEQ ID NO:10);
- Thr-Ile-His-Thr-Asp-Asn-Gly-Ser-Asn (SEQ ID NO:11);
- Thr-Ile-His-Thr-Asp-Asn-Gly-Ser (SEQ ID NO:12);
- Thr-Ile-His-Thr-Asp-Asn-Gly (SEQ ID NO:13);
- Thr-Ile-His-Thr-Asp-Asn (SEQ ID NO:
- 14 );
- Thr-Ile-His-Thr-Asp (SEQ ID NO:15);
- Thr-Ile-His-Thr (SEQ ID NO:16);
- Thr-Ile-His;
- Thr-Ile;
- and
- Thr;
- or a carboxy-terminal COOH or CONH.sub.2 group.
A solid support comprising a solid matrix having an affixed polypeptide, said affixed polypeptide having the formula (SEQ ID NO:21) ##STR10## wherein Xaa is ILE or Leu, and p is an integer having a value of 2 to 6.
Dependencies are inferred from the claim text.