Antibodies to human papillomavirus latent proteins, diagnostic systems and methods
AssigneeThe Scripps Research Institute
InventorsJoakim Dillner, Richard A. Lerner, Richard Smith, D. Elliot Parks
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Abstract
The present invention relates to polypeptides that immunologically mimic papillomavirus latent proteins and to antibodies and monoclonal antibodies that immunoreact with papillomavirus latent proteins. Systems and methods for detecting the presence and type of papillomavirus in a human subject are also described.
Claims37
An antibody composition containing isolated and purified antibody molecules that specifically bind one or more of the following human papillomavirus latent proteins:
- i) the 112 kd diffuse protein,
- ii) the 54 kd filamentous protein,
- iii) the 48 kd filamentous protein,
- iv) the 51 kd nuclear protein, or
- v) the 58 kd nuclear protein.
A hybridoma that produces antibody molecules that specifically bind a latent human papillomavirus protein, said hybridoma selected from the group of hybridomas consisting of ATCC Nos. HB 9718, HB 9719, and HB 9720.
A diagnostic system in kit form for assaying for the presence of a latent human papillomavirus infection in a tissue sample, which system comprises a package containing, in an amount sufficient to perform at least one assay, an anti-polypeptide antibody that specifically binds only one of the polypeptides selected from the group consisting of:
- MADPAGTNGEEGTGC,
- HEDEDKENDGDSLPTC,
- RPFKSNKSTCC,
- CCDWCIAAFGLTPSI,
- TYDSEWQRDQFLSQVKIPC,
- HKSAIVTLTYDSEWQRDQC, and
- CINCQKPLCPEEKQRH.
A diagnostic system in kit form for assaying for the presence of a latent papillomavirus infection in a human subject, which system comprises a package containing, in an amount sufficient to perform at least one assay, an antibody composition containing isolated and purified antibody molecules that specifically bind one or more human papillomavirus latent proteins selected from the group consisting of:
- a) the 112 kd diffuse protein,
- b) the 54 kd filamentous protein,
- c) the 48 kd filamentous protein,
- d) the 51 kd nuclear protein, and
- e) the 58 kd nuclear protein.
An antibody composition containing isolated and purified antibody molecules that specifically bind a latent HPV-related polypeptide, wherein said polypeptide includes an amino acid residue sequence selected from the group consisting of:
- -TYDSE-,
- -LTYDSE-,
- -SAIVTLTYDSE-,
- -HKSAIVTLTYDSE-,
- -HKSAIV-,
- -SAIVTL-,
- -IVTLTYD-,
- -HAIVTVTYDSE-,
- -NAIVTLTYSSE-,
- -NGIVTVTFVTE-,
- -NGIVTVTFUTE-,
- -ILTVT-, and
- -TGILTVTYHSE-.
An antibody composition containing isolated and purified antibody molecules that specifically bind latent HPV-related polypeptide, wherein said polypeptide is selected from the group consisting of:
- MADPAGTNGEEGTGC,
- HEDEDKENDGDSLPTC,
- RPFKSNKSTCC,
- CCDWCIAAFGLTPSI, and
- CINCQKPLCPEEKQRH.
A diagnostic system in kit form for assaying for the presence of a latent papillomavirus infection in a human subject, which system comprises a package containing, in an amount sufficient to perform at least one assay, an antibody composition containing isolated and purified antibody molecules that specifically bind a latent HPV-related polypeptide, wherein said polypeptide includes an amino acid residue sequence selected from the group consisting of:
- -TYDSE-,
- -LTYDSE-,
- -SAIVTLTYDSE-,
- -HKSAIVTLTYDSE-,
- -HKSAIV-,
- -SAIVTL-,
- -IVTLTYD-,
- -HAIVTVTYDSE-,
- -NAIVTLTYSSE-,
- -NGIVTVTFVTE-,
- -NGIVTVTFUTE-,
- -ILTVT-, and
- -TGILTVTYHSE-.
A diagnostic system in kit form for assaying for the presence of a latent papillomavirus infection in a human subject, which system comprises a package containing, in an amount sufficient to perform at least one assay, an antibody composition containing one or more antibody molecules that specifically bind a latent HPV-related polypeptide, wherein said polypeptide includes an amino acid residue sequence selected from the group consisting of:
- MADPAGTNGEEGTGC,
- HEDEDKENDGDSLPTC,
- RPFKSNKSTCC,
- CCDWCIAAFGLTPSI, and
- CINCQKPLCPEEKQRH.
Dependencies are inferred from the claim text.