Device for the direct measurement of low density lipoprotein cholesterol
AssigneeMiles Inc.
InventorsMary M. Foltz, Chen-Jung Hsu, Robert C. Payne, James A. Profitt
Life of this patent
Estimating the term…
Abstract
A dry phase device separates high density lipoprotein (HDL) from a blood (serum or plasma) sample. The device contains a fluid permeable material having dispersed therein finely divided, porous silica or silicate particles as selective absorbant for HDL. By combining the device with a second layer designed to remove very low density lipoproteins/chylomicrons from the blood, and a third layer containing means for quantitative cholesterol detection, there is provided a test device for the direct determination of low density lipoproteins cholesterol.
Claims8
A device for separating high density lipoprotein, very low density lipoprotein and chylomicrons from a blood sample containing high density lipoprotein, low density lipoprotein, very low density lipoprotein and chylomicrons which device comprises a layer of fluid permeable material which has dispersed therein finely divided, porous silica gel particles having a particle size of from 1.mu. to 1000.mu. in their longest dimension and surface pores of from about 80 .ANG. to 1000 .ANG. in diameter, which silica gel particles demonstrate a selective affinity for high density lipoprotein as compared to low density lipoprotein, very low density lipoprotein and chylomicrons and wherein there is dispersed in the layer of fluid permeable material, or in fluid communication with the layer, a reagent system having affinity for very low density lipoproteins and chylomicrons to form a complex with the very low density lipoproteins and the chylomicrons together with means to separate said complex from the blood sample thereby leaving a blood sample substantially free of all lipoprotein but low density lipoprotein.
Dependencies are inferred from the claim text.