Back to results
US 5,399,671 AGranted
Specifically crosslinked hemoglobin with free functionality
OwnerRonald Kluger, Yonghong Songinventors
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
OwnerRonald Kluger, Yonghong Songinventors
Estimating the term…
Hemoglobin is site-specifically crosslinked into its tetrameric form by reaction with a trifunctional reagent which combines electrostatic effects, steric effects and the presence of functional groups so that two of the functional groups react with specific sites on the hemoglobin whilst the third site is left free for reaction with endogenous nucleophilic compounds. A specific example of such a crosslinking reagent is trimesoyl tris(3,5-dibromosalicylate), TTDS, which effects specific crosslinking between the amino groups of lysine-82 on each respective .beta. sub-unit. While the crosslinking reagent TTDS has three available carboxyl groups for the crosslinking reaction, only two so react, leaving one free carboxyl for reaction with exogenous nucleophiles, e.g. to render the hemoglobin product useful as a carrier for nucleophilic compounds through the body's circulatory system.
A process for preparing specifically crosslinked tetrameric hemoglobin by reacting hemoglobin with a chemical crosslinking agent, said crosslinked hemoglobin having crosslinks between the primary amino groups Lys-82 on the respective .beta. sub-units and a free, chemically reactive group derived from the chemical crosslinking reagent, which comprises reacting hemoglobin with a crosslinking reagent corresponding to the general formula
Specifically crosslinked tetrameric hemoglobin having crosslinking groups extending between and covalently bonded to primary amine groups Lys-82 on the respective .beta. sub-units, said crosslinking groups further including a carboxyl group available for reaction with an exogenous nucleophile.
Dependencies are inferred from the claim text.