Interleukin-2 polypeptides
AssigneesAjinomoto Co., Inc., Japanese Foundation for Cancer Research
InventorsTadatsugu Taniguchi, Masami Muramatsu, Haruo Sugano, Hiroshi Matsui, Nobukazu Kashima, Junji Hamuro
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Abstract
Recombinantly produced interleukin-2 exhibits human IL-2 activity, has a molecular weight of about 15,000 daltons, is activity stable at a pH of 2-9 and is resistant to elevated temperatures. The recombinant IL-2 has the principal biological activity of human IL-2, promotion of proliferation of cytotoxic T lymphocytes.
Claims3
An IL-2 polypeptide which is produced by culturing a recombinant microorganism containing a vector having inserted therein a cDNA encoding human interleukin-2, wherein said cDNA is derived from mRNA having a sedimentation coefficient of from 11 to 12 S and is isolated from a human lymphocyte derivative cell capable of producing IL-2, wherein said polypeptide has a biological activity of promotion of proliferation of cytotoxic T lymphocytes, has a molecular weight of about 15,000 daltons, is activity stable over a pH of 2-9, and exhibits a single precipitate when bound by anti-human-IL-2 antibody.
An IL-2 polypeptide which is produced by culturing a recombinant microorganism containing a microbial vector having inserted therein a cDNA having the following base sequence:
- __________________________________________________________________________ GCA CCT ACT TCA AGT TCT ACA AAG AAA ACA CAG CTA CAA CTG GAG CAT TTA CTG CTG GAT TTA CAG ATG ATT TTG AAT GGA ATT AAT AAT TAC AAG AAT CCC AAA CTC ACC AGG ATG CTC ACA TTT AAG TTT TAC ATG CCC AAG AAG GCC ACA GAA CTG AAA CAT CTT CAG TGT CTA GAA GAA GAA CTC AAA CCT CTG GAG GAA GTG CTA AAT TTA GCT CAA AGC AAA AAC TTT CAC TTA AGA CCC AGG GAC TTA ATC AGC AAT ATC AAC GTA ATA GTT CTG GAA CTA AAG GGA TCT GAA ACA ACA TTC ATG TGT GAA TAT GCT GAT GAG ACA GCA ACC ATT GTA GAA TTT CTG AAC AGA TGG ATT ACC TTT TGT CAA AGC ATC ATC TCA ACA CTA ACT. __________________________________________________________________________
An IL-2 polypeptide which is produced by culturing a recombinant microorganism containing a microbial vector having inserted therein a cDNA having the following base sequence:
- __________________________________________________________________________ GCA CCT ACT TCA AGT TCT ACA AAG AAA ACA CAG CTA CAA CTG GAG CAT TTA CTG CTG GAT TTA CAG ATG ATT TTG AAT GGA ATT AAT AAT TAC AAG AAT CCC AAA CTC ACC AGG ATG CTC ACA TTT AAG TTT TAC ATG CCC AAG AAG GCC ACA GAA CTG AAA CAT CTT CAG TGT CTA GAA GAA GAA CTC AAA CCT CTG GAG GAA GTG CTA AAT TTA GCT CAA AGC AAA AAC TTT CAC TTA AGA CCC AGG GAC TTA ATC AGC AAT ATC AAC GTA ATA GTT CTG GAA CTA AAG GGA TCT GAA ACA ACA TTC ATG TGT GAA TAT GCT GAT GAG ACA GCA ACC ATT GTA GAA TTT CTG AAC AGA TGG ATT ACC TTT TGT CAA AGC ATC ATC TCA ACA CTA ACT. __________________________________________________________________________
- harvesting said microorganism cells, obtaining extracts of said cells, and purifying said extracts by:
- salting out with ammonium sulfates, applying said extracts to Sephadex G-15 followed by elution, applying said extracts to DEAE cellulose column chromatography followed by elution, applying said extracts to controlled pore glass beads chromatography followed by elution, applying said extracts to a Sepharose column followed by elution, to yield an IL-2 polypeptide.
Dependencies are inferred from the claim text.