Reduction of background interferences in the molybdate-dye protein assay
AssigneeMiles Inc.
InventorMichael J. Pugia
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Abstract
Disclosed is an improvement to the assay for protein in urine involving the use of a molybdate or tungstate salt and an indicator dye which forms a complex with molybdate or tungstate whose absorption band is shifted in the presence of protein. The improvement involves the use of an ionizable phosphate containing compound characterized by the formula: ##STR1## to reduce background interference caused by constituents normally present in urine.
Claims10
In an assay for the determination of a protein in urine which uses a molybdate or tungstate salt and a dye which forms a complex with molybdate or tungstate ion wherein the absorption band of the complex is shifted in the presence of protein to provide a detectable response, the improvement which comprises introducing an ionizable phosphate containing compound of the formula:
- ##STR6## wherein 2, 3, 4 and 5 are selected from the group consisting of ##STR7## M is H, 1 is any of the above or --O-- and m and n are independently O or 1, in sufficient quantity to reduce background interference while allowing the protein/molybdate/dye or protein/tungstate/dye detectable response to proceed.
A composition of matter suitable for the determination of protein in urine which comprises:
- a) a molybdate or tungstate salt;
- b) a dye which forms a complex with molybdate or tungstate ion whose absorption band is shifted in the presence of protein;
- and
- c) an ionizable phosphate containing compound of the formula:
- ##STR8## wherein 2, 3, 4 and 5 are selected from the group consisting of ##STR9## M is H, 1 is any of the above or --O-- and m and n are independently O or 1.
Dependencies are inferred from the claim text.