Method for assaying enzyme activity using N-acetyl-.beta.-D-glucosamine derivatives
AssigneeNitto Boseki Co., Ltd.
InventorsKatsumasa Kuroiwa, Katsuhiro Katayama, Toshihide Miura, Takeshi Nagasawa
Life of this patent
Estimating the term…
Abstract
Novel N-acetyl-.beta.-D-glucosamine derivatives and a method for assaying N-acetyl-.beta.-D-glucosaminidase activity using the same as substrate are provided. N-Acetyl-.beta.-D-glucosamine derivatives represented by general formula (I): ##STR1## wherein each of R.sub.1 and R.sub.2 independently represents hydrogen atom or methyl or ethyl group, are mixed with a sample containing N-acetyl-.beta.-D-glucosaminidase (NAG) and then absorbance of the reaction solution is determined to assay NAG activity extremely accurately.
Claims6
A method for the determination of N-acetyl-.beta.-D-glucosaminidase activity in a sample which comprises
- mixing a sample containing N-acetyl-.beta.-D-glucosaminidase with an N-acetyl-.beta.-D-glucosamine derivative to form a reaction solution, said derivative defined by the general formula I, ##STR7## wherein each of R.sub.1 and R.sub.2 independently represents a hydrogen or a methyl group or ethyl group;
- and
- determining the activity present by measuring changes in the UV absorbance of the reaction solution at a wavelength of 320-380 nm.
A method for the determination of N-acetyl-.beta.-D-glucosaminidase activity which comprises mixing a human urine sample containing N-acetyl-.beta.-D-glucosaminidase with 6-methyl-2-pyridyl-N-acetyl-1-thio-.beta.-D-glucosaminide to form a reaction solution;
- and measuring UV absorbance of the reaction solution at a wavelength of 320-380 nm.
Dependencies are inferred from the claim text.