Expression of MDR-related gene in yeast cell
AssigneeSuntory Limited
InventorsYuji Shibano, Kazumitsu Ueda, Tohru Komano
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Abstract
A yeast host which can express P-glycoprotein, i.e., the product of MDR-related gene, in the cell membrane in the same state as observed in multidrug resistant cells produced by connecting the MDR-related gene which carries multidrug resistance to a yeast expression vector and transforming the yeast with said recombinant vector; a cell membrane fraction containing a substantial amount of P-glycoprotein produced by said yeast and a process for the preparation thereof; and a recombinant vector for expressing the MDR-related gene in a yeast host.
Claims8
A transformed strain of the yeast saccharomyces cerevisiae having P-glycoprotein accumulated in the cell membrane wherein said yeast has been transformed with a recombinant vector comprising a first DNA fragment consisting essentially of MDR1 gene, a second DNA fragment consisting essentially of PHO5 promoter located upstream of said MDR1 gene, and a third DNA fragment consisting essentially of a yeast GAP terminator located downstream of said MDR1 gene in such a manner as to allow expression of said MDR1 gene in said yeast cell.
Dependencies are inferred from the claim text.