Method for the detection of micrometastases in mesenchymal tissue
AssigneeBoehringer Mannheim GmbH
InventorsHeinz Bodenmueller, Gert Riethmueller
Life of this patent
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Abstract
The present invention concerns a method for the detection of micrometastases of ectodermal or entodermal tumors. Cells from mesenchymal tissue, preferably from bone marrow, are subjected to a fractional lysis. Cytokeratins or fragments thereof are immunologically detected in the cell supernatant.
Claims13
A method of screening for micrometastases of ectodermal or entodermal tumors in blood or bone marrow mesenchymal tissue, comprising the steps of:
- a) collecting mesenchymal tissue cells,
- b) lysing said mesenchymal tissue cells by non-detergent lysis and
- c) detecting cytokeratins or fragments thereof immunologically in any resulting cell supernatant.
The method according to claim 10, wherein R.sub.1 is bound to a solid phase.
A method of screening for micrometastases of ectodermal or entodermal tumors in blood or bone marrow mesenchymal tissue, comprising the steps of:
- a) collecting mesenchymal tissue cells,
- b) incubating the mesenchymal tissue cells in lysis buffer,
- c) separating out any intact cells,
- d) thereafter lysing said intact cells by non-detergent lysis,
- e) centrifuging said cells to remove cell fragments, and
- f) detecting cytokeratins or fragments thereof immunologically in any resulting cell supernatant.
A test kit for screening for micro metastases of ectodermal or entodermal tumors in blood or bone marrow mesenchymal tissue comprising the following components in separate containers:
- a) a first cell lysis reagent containing ammonium chloride for the lysis of erythrocytes,
- b) a second cell lysis reagent for the lysis of nucleated cells, and
- c) a reagent for the immunological detection of cytokeratins or fragments thereof.
Dependencies are inferred from the claim text.