Thermally stable and pH stable subtilisin analogs and method for production thereof
AssigneeAmgen Inc.
InventorsYitzhak Stabinsky, Mark M. Zukowski
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Abstract
A mutated subtilisin suitable for admixture to washing compositions and exhibiting substantially improved stability over naturally occurring Bacillus serine proteases is prepared by expressing a modified gene encoding subtilisin in Bacillus subtilis. A preferred subtilisin analog product differs from wild-type Bacillus alkaline proteases by having any amino acid, and preferably serine, at position 218 in place of asparagine. The product is preferably produced in a strain of B. subtilis which is mutated to block synthesis of endogenous proteases. The method of replacing an Asn or a Gly in an Asn-Gly sequence in order to improve pH and thermal stability may be applied to other sites in subtilisin and to other proteins as well.
Claims9
An enzymatically active analog of a Bacillus subtilisin, said Bacillus subtilisin having an amino acid sequence comprising an Asn-Gly sequence, said analog having an amino acid sequence wherein an asparaginyl residue in said Asn-Gly sequence of said Bacillus subtilisin is replaced by a residue of a different amino acid.
An enzymatically active analog of a Bacillus subtilisin wherein said enzymatically active analog has an amino acid sequence wherein an asparaginyl residue naturally occurring at a position corresponding to position 218 in the amino acid sequence disclosed in FIG. 6 is replaced by a residue of a different amino acid.
Dependencies are inferred from the claim text.