Method for generation of antibodies to cell surface molecules
AssigneeCetus Oncology Corporation
InventorsMark De Boer, Leah B. Conroy
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method of generating antibodies directed against cell surface antigens. The method uses as immunogen recombinant insect cells into which have been transfected coding regions for a molecule containing a cell surface antigen. Host animals are immunized with these transfected insect cells to generate antibodies directed against the cell surface antigen. Antibody-producing cells from the host animal are used to generate monoclonal antibody-producing hybridoma cells. Sera and hybridoma supernatants can be tested for the presence of antibodies against the surface antigen, using the transfected cells in a screening assay.
Claims17
A method of generating an immortalized cell line producing a monoclonal antibody specific against a selected membrane-associated antigen, comprising
- producing the membrane-associated antigen on the cell surface of insect cells,
- injecting said insect cells into a host animal,
- recovering from the host animal cells capable of producing antibody molecules,
- immortalizing such cells for growth in cell culture,
- screening the immortalized cells for production of antibodies specific to the membrane-associated antigen in a binding assay employing non-insect cells having the membrane-associated antigen on the surface of the non-insect cells, and
- selecting those immortalized cells producing antibodies which bind to the membrane-associated antigen on the surface of said non-insect cells.
A method of producing sera containing antibodies specific against a selected membrane-associated antigen, comprising
- producing the membrane-associated antigen on the cell surface of insect cells,
- injecting said insect cells into a host animal, and
- screening sera from said animal for production of antibodies specific to the membrane-associated antigen in a binding assay employing non-insect cells having the membrane-associated antigen on the cell surface.
Dependencies are inferred from the claim text.