Azido-substituted fatty acid analog enzyme substrates
AssigneeWashington University
InventorsBalekudru Devadas, Jeffrey I. Gordon, Steven P. Adams
Life of this patent
Estimating the term…
Abstract
Azido-substituted fatty acid analogs which are useful in the fatty acid acylation of peptides and proteins and as antiviral agents are disclosed having the following chemical structure: Z--(CH.sub.2).sub.x COOR wherein Z=azido, tetrazolyl or triazolyl R=H or C.sub.1 -C.sub.8 alkyl, and x=8-12.
Claims6
A method of acylating a peptide or protein to form a N-myristoylated type peptide or protein, respectively, in a N-myristoyltransferase assay comprising reacting said peptide or protein with N-myristoyltransferase and an enzyme substrate consisting of the CoA ester of a fatty acid analog of myristic acid having the following structure:
- Z--(CH.sub.2).sub.x COOR
- wherein
- Z=azido, tetrazolyl or triazolyl
- R=H or C.sub.1 -C.sub.8 alkyl, and
- x=8-12.
Dependencies are inferred from the claim text.