Identification of plant-responsive genes of bacteria

A method for identifying plant responsive genes of bacteria, comprising:

• (a) mutating the genome of an initial bacterial strain with a transposon to produce transposon-insertion mutants, wherein said transposon is a promoter probe transposon designated TnCIB116 and comprises a promoterless marker gene;
• (b) exposing the bacterial mutants to a plant-derived substance;
• (c) identifying which of the genomic mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plant,derived substance;
• and
• (d) identifying the gene that is associated with the promoter identified in step (c) in the wild-type bacterial strain, wherein the identified gene is thus identified as a plant responsive gene.

A method for identifying plant responsive genes of bacteria, comprising:

• (a) mutating the genome of an initial bacterial strain with a transposon to produce transposon-insertion mutants, said transposon comprising a promoterless marker gene, wherein the marker gene is selected from the group consisting of an ice-nucleation gene, the marker gene in plasmid pCIB116, and the marker gene in plasmid pCIB3330;
• (b) exposing the bacterial mutants to plants;
• (c) identifying which of the genomic mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plants;
• and
• (d) identifying the gene that is associated with the promoter identified in step (c) in the wild-type bacterial strain, wherein the identified gene is thus identified as a plant responsive gene.

A method for identifying plant responsive genes of bacteria, comprising:

• (a) mutating the genome of an initial bacterial strain with a transposon to produce transposon-insertion mutants, wherein said transposon is a promoter probe transposon designated TnCIB116 and comprises a promoterless marker gene;
• (b) exposing the bacterial mutants to plants;
• (c) identifying which of the genomic mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plants;
• and
• (d) identifying the gene that is associated with the promoter identified in step (c) in the wild-type bacterial strain, wherein the identified gene is thus identified as a plant responsive gene.

A method for cloning plant responsive promoters of bacteria, comprising:

• (a) mutating the genome of an initial bacterial strain with a transposon to produce transposon-insertion mutants, said transposon comprising a promoterless marker gene, wherein the marker gene is selected from the group consisting of an ice-nucleation gene, the marker gene in plasmid pCIB116, and the marker gene in plasmid pCIB3330;
• (b) exposing the bacterial mutants to a plant-derived substance;
• (c) identifying which of the genomic mutants have a transposon-associated promoter responsive by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plant-derived substance;
• and
• (d) cloning the responsive promoter from a mutant identified in step (c).

A method for cloning plant responsive promoters of bacteria, comprising:

• (a) mutating the genome of an initial bacterial strain with a transposon to produce transposon-insertion mutants, wherein said transposon is a promoter probe transposon designated TnCIB116 and comprises a promoterless marker gene;
• (b) exposing the bacterial mutants to a plant-derived substance;
• (c) identifying which of the genomic mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plant-derived substance;
• and
• (d) cloning the responsive promoter from a mutant identified in step (c).

A method for obtaining bacterial mutants with marked plant responsive genes, comprising:

• (a) mutating the genome of an initial bacterial strain with a transposon to produce transposon-insertion mutants, said transposon comprising a promoterless marker gene, wherein the marker gene is selected from the group consisting of an ice-nucleation gene, the marker gene in plasmid pCIB116, and the marker gene in plasmid pCIB3330;
• (b) exposing the bacterial routants to a plant-derived substance;
• and
• (c) identifying which of the bacterial mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plant-derived substance.

A method for obtaining bacterial mutants with marked plant responsive genes, comprising:

• (a) mutating the genome of an initial bacterial strain with a transposon to produce transposon-insertion mutants, wherein said transposon is a promoter probe transposon designated TnCIB116 and comprises a promoterless marker gene;
• (b) exposing the bacterial mutants to a plant-derived substance;
• and
• (c) identifying which of the bacterial mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plant-derived substance.

A method for identifying a Pseudomonas gene responsive to a plant host, comprising:

• (a) mutating the genome of an initial Pseudomonas strain with a transposon to produce transposon-insertion mutants, said transposon comprising a promoterless marker gene, wherein the marker gene is selected from the group consisting of an ice-nucleation gene, the marker gene in plasmid pCIB116, and the marker gene in plasmid pCIB3330;
• (b) exposing the Pseudomonas mutants to a plant-derived substance;
• (c) identifying which of the Pseudomonas mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plant-derived substance;
• and
• (d) identifying the gene that is associated with the promoter identified in step (c) in the wild-type Pseudomonas strain, wherein the identified gene is thus identified as a plant responsive gene.

A method for identifying a Pseudomonas gene responsive to a plant host, comprising:

• (a) mutating the genome of an initial Pseudomonas strain with a transposon to produce transposon-insertion mutants, wherein said transposon is a promoter probe transposon designated TnCIB116 and comprises a promoterless marker gene;
• (b) exposing the Pseudomonas mutants to a plant-derived substance;
• (c) identifying which of the Pseudomonas mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plant-derived substance;
• and
• (d) identifying the gene that is associated with the promoter identified in step (c) in the wild-type Pseudomonas strain, wherein the identified gene is thus identified as a plant responsive gene.

A method for identifying a Pseudomonas gene responsive to a plant host, comprising:

• (a) mutating the genome of an initial Pseudomonas strain with a transposon to produce transposon-insertion mutants, said transposon comprising a promoterless marker gene, wherein the marker gene is selected from the group consisting of an ice-nucleation gene, the marker gene in plasmid pCIB116, and the marker gene in plasmid pCIB3330;
• (b) exposing the Pseudomonas mutants to plants;
• (c) identifying which of the Pseudomonas mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plants;
• and
• (d) identifying the gene that is associated with the promoter identified in step (c) in the wild-type Pseudomonas strain, wherein the identified gene is thus identified as a plant responsive gene.

A method for identifying a Pseudomonas gene responsive to a plant host, comprising:

• (a) mutating the genome of an initial Pseudomonas strain with a transposon to produce transposon-insertion mutants, wherein said transposon is a promoter probe transposon designated TnCIB116 and comprises a promoterless marker gene;
• (b) exposing the Pseudomonas mutants to plants;
• (c) identifying which of the Pseudomonas mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plants;
• and
• (d) identifying the gene that is associated with the promoter identified in step (c) in the wild-type Pseudomonas strain, wherein the identified gene is thus identified as a plant responsive gene.

A method for cloning a Pseudomonas promoter with altered responsiveness to a plant, comprising:

• (a) mutating the genome of an initial Pseudomonas strain with a transposon to produce transposon-insertion mutants, said transposon comprising a promoterless marker gene, wherein the marker gene is selected from the group consisting of an ice-nucleation gene, the marker gene in plasmid pCIB116, and the marker gene in plasmid pCIB3330;
• (b) exposing the Pseudomochas mutants to a plant-derived substance;
• (c) identifying which of the Pseudomonas mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence Of the plant-derived substance;
• and
• (d) cloning the responsive promoter from a mutant identified in step (c).

A method for cloning a Pseudomonas promoter with altered responsiveness to a plant, comprising:

• (a) mutating the genome of an initial Pseudomonas strain with a transposon to produce transposon-insertion mutants, wherein said transposon is a promoter probe transposon designated TnCIB116 and comprises a promoterless marker gene;
• (b) exposing the Pseudomonas mutants to a plant-derived substance;
• (c) identifying which of the Pseudomonas mutants have a transposon-associated promoter inducible by the plant-derived substance by comparing expression of the marker gene in the presence and absence of the plant-derived substance;
• and
• (d) cloning the responsive promoter from a mutant identified in step (c).