Curculin C

Curculin C, having interchain disulfide linkages between the 77th cysteine residues of two monomer chains and between the 109th cysteine residues of said two monomer chains, thus giving a dimer, each said monomer chain having an amino acid sequence as specified in SEQ ID No:i, and each said monomeric chain having an intrachain disulfide linkage between the 29th cysteine residue and the 52nd cysteine residue, said curculin C being obtained by extracting Curculigo latifolia fruits or sarcosarp thereof with an aqueous salt solution, thereafter salting out a crude extract from the salt solution and subjecting the resultant precipitate to purification by CM-Sepharose ion exchange chromatography followed by gel filtration chromatography with the use of a Sephadex G-100 column, said curculin C being characterized by a single peak being obtained when said curculin C is separated with the use of a Sephadex G-100 column and 0.01M phosphate buffer solution of pH 6.8, containing 0.5M NaCl .