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US 5,395,757 AGranted
Method for the hydrolysis of sugar compounds and reagent therefor
AssigneeTakara Shuzo Co., Ltd.
InventorsKumi Hayakawa, Mutsumi Sano, Ikunoshin Kato
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Abstract
A method for the hydrolysis of only the lacto-N-biosidic (Gal.beta.1-3GlcNAc.beta.1-) linkages at the non-reducing termini of sugar compounds, characterized by the use of glycosidase that is specific for only the lacto-N-biosidic linkage in said sugar compounds. And disclosed is a reagent for use in hydrolysis of only the lacto-N-biosidic linkage at the non-reducing termini of sugar compounds.
Claims2
An isolated and purified lacto-N-biosidase having the following properties:
- (1) action:
- hydrolyzes an oligosaccharide of formula (i) specifically at the arrow-marked site:
- ##STR3## wherein R represents a sugar residue which is optionally labeled with fluorescent label at the reducing end;
- (2) substrate specificity:
- hydrolyzes a sugar compound of formula (ii) specifically at the arrow-marked site:
- ##STR4## but does not hydrolyze an oligosaccharide described by formula (iii):
- Gal.beta.1--4GlcNAc.beta.1--R' (iii)
- wherein R' represents a sugar residue which is optionally labeled with fluorescent label at the reducing end;
- (3) optimal pH:
- 5.5-6.0;
- (4) pH stability:
- stable in the pH range of 4.0-7.0 when kept at 4.degree. C. for 16 hours;
- and
- (5) optimal temperature:
- 40.degree. C.-55.degree. C.;
- (6) obtained from Streptomyces sp 142.
13 more elements
Dependencies are inferred from the claim text.