Production of acidic FGF protein

A vector comprising (1) a cDNA sequence encoding a human acidic fibroblast growth factor (haFGF) or a deletion-type mutein of haFGF which is Selected from the group consisting of 1, 5, 8, 9, 11, 12, 20 and 43 amino terminal residue-deleted haFGF and (2) a T7 promoter which is operably linked into an upstream site therefrom.

A transformant host cell containing a vector comprising (1) a cDNA sequence encoding a human acidic fibroblast growth factor (haFGF) or a deletion-type mutein of haFGF which lacks up to 43 amino acids from the N-terminus of haFGF and (2) a T7 promoter which is operably linked into an upstream site therefrom.

A method for the preparation of a human acidic fibroblast growth factor (haFGF) or a deletion-type mutein of haFGF which lacks up to 43 amino acids from the N-terminus of haFGF which comprises culturing a transformant host cell containing a vector comprising (1) a cDNA sequence encoding an haFGF or said deletion-type mutein and (2) a T7 promoter which is operably linked into an upstream site therefrom in a culture medium to produce and accumulate an haFGF or said deletion-type mutein in the transformed cells, lysing the cells and purifying the protein from resulting haFGF-containing solution or said deletion-type mutein-containing solution.