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US 5,395,755 AGranted
Antioxidant assay
AssigneeBritish Technology Group Limited
InventorsGary H. G. H. Thorpe, Thomas P. Whitehead
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Abstract
A method of assay of antioxidant activity of a biological fluid, which comprises monitoring the change exerted by the sample on a progressing luminescent reaction.
Claims11
A method of assay of the antioxidant activity of a sample suspected of having such activity, which method comprises the steps of:
- (a) initiating a chemiluminescent reaction and allowing said reaction to progress, thereby to generate a level of luminescence, said level being selected from the group consisting of (i) a rising level between 90 to 100% of maximum, (ii) the maximum, and (iii) a post-maximum substantially constant plateau level;
- (b) adding said sample to said progressing chemiluminescent reaction, said sample causing said level of luminescence generated by said reaction to change when said sample has antioxidant activity;
- (c) monitoring said change in the level of luminescence;
- and
- (d) determining the antioxidant activity of said sample assayed by reference to that of samples of known antioxidant activity subjected to steps (a) to (c) above.
2 more elements
Dependencies are inferred from the claim text.