Back to results
US 5,395,753 AGranted
Method for diagnosing rheumatoid arthritis
AssigneeTheratech, Inc.
InventorRamesh K. Prakash
Life of this patent
Estimating the term…
Abstract
A method is described for diagnosing rheumatoid arthritis by providing a recombinant IgM-specific rheumatoid arthritis-associated antigen and detecting antibodies against the antigen in patient sera. Preliminary steps of making a cDNA library from polyadenylated RNA purified from human cells, selecting recombinants that express the antigen, recloning cDNA containing the antigen gene in a high level expression vector, expressing the antigen in transformed cells, and purifying the antigen are also described.
Claims23
A method for diagnosing rheumatoid arthritis comprising the steps of:
- (a) providing a recombinant RAMA antigen that is specifically detectable by rheumatoid arthritis-associated IgM antibodies;
- (b) incubating said RAMA antigen with patient serum to form a complex of said RAMA antigen and said IgM antibodies;
- and
- (c) detecting said complex as a measure of said IgM antibodies against said RAMA antigen in patient sera.
1 more element
Dependencies are inferred from the claim text.