Long emission wavelength chemiluminescent compounds and their use in test assays
AssigneesCiba Corning Diagnostics Corp., Ciba-Geigy Corporation
InventorsSay-Jong Law, Qingping Jiang, Walter Fischer, John T. Unger, Elizabeth K. Krodel
Life of this patent
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Abstract
An assay method incorporating at least two different chemiluminescent compounds for detection and/or quantitation of at least two substances in a test sample is described. The synthesis of chemiluminescent reagents or conjugates for use in such methods as well as kits incorporating such reagents are also disclosed. The assays have particular application in the field of clinical diagnostics.
Claims26
A detection and/or quantitation method for at least two substances in a test sample comprising:
- (a) providing at least two different chemiluminescent compounds, wherein at least one of said compounds includes a linear aromatic four-ring fused acridinium compound, and another includes an angular aromatic four-ring fused acridinium compound or a three-ring acridinium compound, each of said compounds having conjugated thereto a molecule specific for a test substance in said test sample so that a reaction will occur between said conjugated molecule and test substance;
- and
- (b) simultaneously detecting the emission signals of said chemiluminescent compounds, wherein said signals are utilized to detect and/or quantitate the said test substances.
An amplification method for target sequences in a test sample comprising:
- (a) providing a test sample suspected of containing one or more target sequences;
- (b) adding an internal reference or control to said test sample;
- (c) amplifying the target sequences;
- (d) providing at least two different chemiluminescent conjugates, each chemiluminescent conjugate having bound thereto a molecule complementary to one of said target sequence, internal reference or control, such that a detectable product is produced;
- wherein at least one conjugate includes a linear aromatic four-ring fused acridinium compound and another includes an angular aromatic four-ring fused acridinium compound or a three-ring acridinium compound;
- and
- (e) simultaneously detecting and/or quantitating amplified target sequences and the internal reference or control by emissions of said chemiluminescent conjugates.
An amplification method for target sequences in a test sample comprising:
- (a) providing a test sample suspected of containing one or more target sequences;
- (b) amplifying the target sequences;
- (c) providing at least two different chemiluminescent conjugates, each chemiluminescent conjugate having bound thereto a molecule complementary with a target sequence(s) such that a detectable product is produced and wherein at least one conjugate includes a linear aromatic ring fused acridinium compound and another includes an angular aromatic ring fused acridinium compound or an acridinium compound;
- and
- (d) simultaneously detecting and/or quantifying amplified target sequences by emissions of said chemiluminescent conjugates.
A chemiluminescent label assay method for detection and/or quantitating at least two substances in a test sample comprising:
- (a) providing a first chemiluminescent reagent to the test sample, said first chemiluminescent reagent including a benzacridinium four-ring system and which is specific to one test substance in said test sample;
- (b) providing a second chemiluminescent reagent to the test sample, said second chemiluminscent reagent including a three-ring acridinium ring system and which is specific to another test substance in said test sample;
- and
- (c) activating said chemiluminescent reagents to provide discernable emission signals for detecting and/or quantitating the test substances.
Dependencies are inferred from the claim text.