Assay for cytotoxic T cells
AssigneeUnited Biomedical, Inc.
InventorsAndrew J. McMichael, Douglas F. Nixon, Frances M. Gotch
Life of this patent
Estimating the term…
Abstract
A method of detecting in a sample CTL specific for a particular virus, e.g. HIV, comprises contacting the sample with a support carrying immobilized antibodies to a surface antigen on T cells; separating the support and attached materials; contacting the separated support with target cells matched to the HLA type of the source of the sample and with HLA matched peptide epitope of the virus that interacts with the CTL of interest; and monitoring lysis of the target cells.
Claims7
A method of detecting in a sample the presence of cytotoxic T lymphocytes specific for a virus, comprising contacting said sample with a support carrying immobilized monoclonal antibodies to a CD8 surface antigen on T cells;
- separating said support and attached materials from the rest of said sample;
- contacting the separate support with target cells matched to the HLA type of the source of the sample and with an HLA matched peptide epitope of the virus that interacts with a cytotoxic T lymphocyte;
- and monitoring lysis of said target cells.
Dependencies are inferred from the claim text.