Surface plasmon resonance detector having collector for eluted ligate
AssigneeSmithKline Beecham Corporation
InventorsMichael Brigham-Burke, Daniel J. O'Shannessy
Life of this patent
Estimating the term…
Abstract
An improved surface plasmon resonance detector, capable of recovering a desired eluted ligate allows for the collection and subsequent analysis of ligate solutions following interaction of the ligate with an immobilized ligand on a surface of a sensor chip of the surface plasmon resonance detector, permits the isolation and characterization of novel ligates and ligands.
Claims5
A surface plasmon resonance detector comprising a surface plasmon resonance sensor chip, optical means for directing light toward said sensor chip and for monitoring the resonance angle by detecting light reflected from said sensor chip, and first means for conducting a ligate solution to and from said sensor chip, said detector further comprising
- means providing a receptacle for recovering eluted ligate;
- wherein said means for conducting a ligate solution to and from said sensor chip is a microfluidic cartridge in contact with said sensor chip and coupled to a connector block having second means for conducting eluted ligate to said receptacle;
- wherein said connector block further comprises an injection port, an open channel, a drain port in communication with said open channel, and a drain passage leading from said microfluidic cartridge and having an end opening into said open channel, said second conducting means to said receptacle fits into said end opening and leads to said receptacle;
- wherein a sample of ligate solution is injected into said injection port and delivered to said sensor chip through said first conducting means, and eluted ligate flows from said sensor chip through said second means for conducting eluted ligate into said receptacle, whereby the eluted ligate can be recovered for subsequent analysis.
A surface plasmon resonance detector comprising a surface plasmon resonance sensor chip, optical means for directing light toward said sensor chip and for monitoring the resonance angle by detecting light reflected from said sensor chip, and means for conducting a ligate solution to and from said sensor chip,
- wherein said means for conducting a ligate solution to and from said sensor chip is a microfluidic cartridge in contact with said sensor chip and a connector block having means for transferring eluted ligate to a selected instrument, coupled to said microfiuidic cartridge;
- wherein said connector block further comprises an injection port, an open channel, a drain port in communication with said open channel, and a drain passage leading from said microfiuidic cartridge and having an end opening into said open channel said transferring means to said instrument fits into said end opening and leads to said instrument;
- wherein a sample of ligate solution is injected into said injection port and delivered to said sensor chip through said conducting means, and eluted ligate flows from said sensor chip to said selected instrument through said transferring means whereby the eluted ligate can be subsequently analyzed.
Dependencies are inferred from the claim text.