Analogs of human granulocyte-macrophage colony stimulating factor
AssigneeImmunex Corporation
InventorsMichael C. Deeley, Virginia L. Price
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Abstract
Amplified expression of recombinant DNA products is achieved in hosts expressing proteases that cleave at multi-basic amino acid residues. To this end, cDNAs encoding granulocyte-macrophage colony stimulating factor (GM-CSF) are mutated such that one or both of the arginine residues at positions 23 and 24 of the protein product are deleted or replaced by non-basic amino acid residues. The GM-CSF analogs thus obtained maintain the activity of the wild-type protein.
Claims8
A polypeptide possessing human granulocyte-macrophage colony stimulating activity and comprising a sequence of amino acids which is identical to that shown as Ala.sup.1 to Glu.sup.127 in FIG. 1A but for the deletion or replacement by a non-basic amino acid of at least one of Arg.sup.23 and Arg.sup.24.
Dependencies are inferred from the claim text.