DNA, vectors and transformed hosts encoding Trichoderma reesei endoglucanase I
AssigneeOy Alko AB
InventorsJonathan Knowles, Merja Penttila, Tuula Teeri, Helena M. K. Nevalainen, Paivi Lehtovaara-Helenius
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Abstract
Methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes, achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.
Claims16
A substantially pure DNA molecule comprising DNA encoding a polypeptide comprising the mature endoglucanase I protein amino acid sequence shown in FIG. 6 or FIG. 11.
A substantially pure DNA molecule comprising the cDNA sequence shown in FIG. 6 or FIG. 11, encoding a polypeptide comprising the mature endoglucanase I protein amino acid sequence shown in FIG. 6 or FIG. 11.
A substantially pure DNA molecule encoding an amino acid sequence comprising the endoglucanase I signal sequence shown in FIG. 6 or FIG. 11, said endoglucanase I signal DNA sequence encoding the peptide
- MetAlaProSerValThrLeuProLeuThrThrAlaIleLeuAlaIleAlaArgLeuValAlaAla.
Plasmid pTT11.
E. coli host ALKO2296 having deposit number DSM6873.
Dependencies are inferred from the claim text.